Magnetic resonance-imaging of the effect of targeted antiangiogenic gene delivery in a melanoma tumour model
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- 作者：Walter Hundt ; Silke Steinbach ; Dirk Mayer ; Mykhaylo Burbelko…
- 关键词：Malignant melanoma ; Gene delivery systems ; Magnetic resonance imaging ; Perfusion magnetic resonance imaging ; Nanoparticle
- 刊名：European Radiology
- 出版年：2015
- 出版时间：April 2015
- 年：2015
- 卷：25
- 期：4
- 页码：1107-1118
- 全文大小：1,047 KB
- 参考文献：1. van der Schaft DWJ, Ramkrishnan S, Molema G, Griffioen AW in: G. Molema, D.K.F. Meijer (eds),(2001) Drug Targeting: Organ-Specific Strategies, Wiley-VCH, Weinheim M 233-54
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14. Mayr, NA, Yuh, WT, Magnotta, VA, Ehrhardt, JC, Wheeler, JA, Sorosky, JI, Davis, CS, Wen, BC, Martin, DD, Pelsang, RE, Buller, RE, Oberley, LW, Mellenberg, DE, Hussey, DH (1993) Tumor perfusion studies using fast magnetic resonance imaging technique in advanced cervical cancer: a new noninvasive predictive assay. Int J Radiat Oncol Biol Phys 6: pp. 623-633
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17. Kloeckner, R, Otto, G, Biesterfeld, S, Oberholzer, K, Dueber, C, Pitton, MB (2010) MDC - 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Imaging and Radiology
Diagnostic Radiology
Interventional Radiology
Neuroradiology
Ultrasound
Internal Medicine - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-1084
文摘
Objectives We investigated the effect of targeted gene therapy to melanoma tumours (M21) by MR-imaging. Methods M21 and M21-L tumours were grown to a size of 850 mm3. M21 and M21-L tumours were intravenously treated with an αvβ3-integrin-ligand-coupled nanoparticle (RGDNP)/RAF(-) complex five times every 72 hours. MRI was performed at set time intervals 24h and 72h after the i.v. injection of the complex. The MRI protocol was T1-wt-SE±CM, T2-wt-FSE, DCE-MRI, Diffusion-wt-STEAM-sequence, T2-time obtained on a 1.5-T-GE-MRI device. Results The size of the treated M21 tumours kept nearly constant during the treatment phase (847.8±31.4 mm3 versus 904.8±44.4 mm3). The SNR value (T2-weighted images) of the tumours was 36.7±0.6 and dropped down to 30.6±1.9 (p=0.004). At the beginning the SNR value (T1-weighted images) of the tumours after contrast medium application was 42.3±1.9 and dropped down to 28.5±3.0 (p-3 mm2/s versus 0.67±0.04x10-3 mm2/s). The DCE-MRI showed a reduction of the slope and of the Akep of 67.8±4.3 % respectively 64.8±3.3 % compared to baseline. Conclusions Targeted gene delivery therapy induces significant changes in MR-imaging. MRI showed a significant reduction of contrast medium uptake parameters and increase of the diffusion coefficient of the tumours. Key point -Treatment with targeted gene-delivery therapy can be monitored by MR imaging -DCE and diffusion-weighted imaging are appropriate methods for monitoring this therapy -Functional changes are significant prior to any morphological changes