Preparation and Application of Modified VEGFR-2 Cell Membrane Chromatographic Separation System

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• 作者：Ying Wang ; Simeng Fang ; Guisen Zhao ; Weihong Wang ; Zhongxi Zhao
• 刊名：Chromatographia
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：79
• 期：11-12
• 页码：675-684
• 全文大小：1,205 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112
• 卷排序：79

文摘

The vascular endothelial growth factor receptor-2 (VEGFR-2) in some tumor cells is a significant target for drug discovery. In this work, a modified model of VEGFR-2 cell membrane stationary phase (CMSP) was prepared by immobilizing U251 cell membrane onto the surface of chitosan-silica (CTS-SiO₂) hybrid carrier. The surface and chromatographic characteristics of VEGFR-2 CMSP were studied. We have developed modified VEGFR-2 cell membrane chromatography for screening drugs and sunitinib malate was used as a positive control. The interaction between the new compounds and membrane receptor was determined by the capacity factors (kʹ). The in vitro cytotoxicity of 10 new compounds on U251 cell viability was determined by MTT test separately to verify the potential pharmacological activity. The modified VEGFR-2 cell membrane chromatographic system demonstrated fast and effective characteristics for screening leading compounds.KeywordsVEGFR-2 cell membrane chromatographyChitosan-silicaHigh performance liquid chromatographyScreening new compoundsSunitinib