Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy

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• 作者：Rong Dong (1) (2)
  Jinhua Long (3)
  Xiaoli Xu (1) (2)
  Chunlin Zhang (2)
  Zongyao Wen (5)
  Long Li (4)
  Weijuan Yao (5)
  Zhu Zeng (1) (2) (6)
  
• 关键词：Dendritic cell ; Fourier transformed infrared spectroscopy ; Functional states of cells ; NF ; κB
• 刊名：BioMedical Engineering OnLine
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：13
• 期：1
• 全文大小：265 KB
• 作者单位：Rong Dong (1) (2)
  Jinhua Long (3)
  Xiaoli Xu (1) (2)
  Chunlin Zhang (2)
  Zongyao Wen (5)
  Long Li (4)
  Weijuan Yao (5)
  Zhu Zeng (1) (2) (6)
  
  1. Department of Biomedical Engineering, Guiyang Medical College, Guiyang, Guizhou Province, 550004, People’s Republic of China
  2. Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou Province, 550004, People’s Republic of China
  3. Department of Head and Neck, Affiliated Cancer Hospital, Guiyang Medical College, Guiyang, Guizhou Province, 550004, People’s Republic of China
  5. Center of Hemorheology, School of Basic Medical Sciences, Health Science Center of Peking University, Beijing, 100083, People’s Republic of China
  4. Department of Nephrology, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou Province, 550004, P. R. China
  6. Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province, 550004, P. R. China
  
• ISSN：1475-925X

文摘

Background Dendritic cells are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses. The dendritic cell-based vaccination against cancer has been clinically achieved promising successes. But there are still many challenges in its clinical application, especially for how to identify the functional states. Methods The CD14+ monocytes were isolated from human peripheral blood after plastic adherence and purified to approximately 98% with cocktail immunomagnetic beads. The immature dendritic cells and mature dendritic cells were induced by traditional protocols. The resulting dendritic cells were cocultured with normal cells and cancer cells. The functional state of dendritic cells including immature dendritic cells (imDCs) and mature dendritic cells (mDCs) under different conditioned microenvironments were investigated by Fourier transformed infrared spectroscopy (FTIR) and molecular biological methods. Results The results of Fourier transformed infrared spectroscopy showed that the gene transcription activity and energy states of dendritic cells were specifically suppressed by tumor cells (P-lt;-.05 or 0.01). The expression levels of NF-kappa B (NF-κB) in dendritic cells were also specifically inhibited by tumor-derived factors (P-lt;-.05 or 0.01). Moreover, the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were closely correlated with the expression levels of NF-κB (R2:0.69 and R2:0.81, respectively). Conclusion Our results confirmed that the ratios of absorption intensities of Fourier transformed infrared spectroscopy at given wave numbers were positively correlated with the expression levels of NF-κB, suggesting that Fourier transformed infrared spectroscopy technology could be clinically applied to identify the functional states of dendritic cell when performing dendritic cell-based vaccination. It’s significant for the simplification and standardization of dendritic cell-based vaccination clinical preparation protocols.