Visual characterization of targeted effect of holo-transferrin-tagged dihydroartemisinin on human breast cancer cells
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- 作者：WeiLing Xie (1)
PeiHui Yang (1) (2)
Xin Zeng (1)
Hui Wang (1)
HuaiHong Cai (1)
JiYe Cai (1) (2) - 关键词：dihydroartemisinin ; holo ; transferrin ; breast cancer cells ; atomic force microscopy ; cytotoxicity
- 刊名：Chinese Science Bulletin
- 出版年：2010
- 出版时间：August 2010
- 年：2010
- 卷：55
- 期：22
- 页码：2390-2395
- 全文大小：952KB
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PeiHui Yang (1) (2)
Xin Zeng (1)
Hui Wang (1)
HuaiHong Cai (1)
JiYe Cai (1) (2)
1. Department of Chemistry, Jinan University, Guangzhou, 510632, China
2. Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China - ISSN：1861-9541
文摘
Targeted drugs could significantly reduce cytotoxic effect and increase therapeutic activity. Dihydroartemisinin (DHA) has been shown to be effective in killing cancer cells. However, it exhibits non-targeted property. Holo-transferrin (TF) is a suitable drug-carrier to target cancer cells, because cancer cells need iron uptake by the TF-mediated mechanism to maintain their uncon-trolled growth. Furthermore, TF receptors (TF-R) are highly expressed on cancer cell surfaces. In this paper, 3-(4,5-dimethylthi-azol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the different killing effect of 4-(12-Dihydroart-emisininoxy) Benzoic Acid Hydrozide-transferrin (DBAH-TF) on human breast cancer cells (MCF-7) cells and human normal breast (HNB) cells, and atomic force microscopy (AFM) was used to visually observe the targeted effect of DBAH-TF on MCF-7 cells. MTT results show that DBAH-TF is 172 times more potent than DHA in killing MCF-7 cells, while the cytotoxic effect of DBAH-TF on HNB cells is merely 1/33 to DHA. Also, the killing effect of DBAH-TF on MCF-7 cells is 286 times that on HNB cells, showing targeted effect. Moreover, there are distinct differences in ultrastructures of cellular surfaces after DBAH-TF and DHA treatment. Through AFM imaging, many characteristic holes were observed on the cancer cell surface after being effected by DBAH-TF, which differ from the holes with irregular shapes affected by DHA. These results visually show that the DBAH-TF targeted drug has more potent killing effect on MCF-7 cells compared with DHA.