Coenzyme Q10 ameliorates oxidative stress and prevents mitochondrial alteration in ischemic retinal injury
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- 作者：Dongwook Lee (1) (2)
Keun-Young Kim (3)
Myoung Sup Shim (1)
Sang Yeop Kim (1)
Mark H. Ellisman (3)
Robert N. Weinreb (1)
Won-Kyu Ju (1) - 关键词：Coenzyme Q10 ; Retinal ischemia ; Retinal ganglion cell ; Oxidative stress ; Mitochondrial transcription factor A ; Mitochondrial DNA
- 刊名：Apoptosis
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：19
- 期：4
- 页码：603-614
- 全文大小：1,514 KB
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Keun-Young Kim (3)
Myoung Sup Shim (1)
Sang Yeop Kim (1)
Mark H. Ellisman (3)
Robert N. Weinreb (1)
Won-Kyu Ju (1)
1. Laboratory for Optic Nerve Biology, Department of Ophthalmology, Hamilton Glaucoma Center, University of California San Diego, 9415 Campus Point Drive, La Jolla, CA, 92037, USA
2. Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute, Chonbuk National University Hospital, Chonju, Chonbuk, Republic of Korea
3. Department of Neuroscience, Center for Research on Biological Systems, National Center for Microscopy and Imaging Research, University of California San Diego, La Jolla, CA, USA - ISSN：1573-675X
文摘
Coenzyme Q10 (CoQ10) acts by scavenging reactive oxygen species for protecting neuronal cells against oxidative stress in neurodegenerative diseases. We tested whether a diet supplemented with CoQ10 ameliorates oxidative stress and mitochondrial alteration, as well as promotes retinal ganglion cell (RGC) survival in ischemic retina induced by intraocular pressure elevation. A CoQ10 significantly promoted RGC survival at 2?weeks after ischemia. Superoxide dismutase 2 (SOD2) and heme oxygenase-1 (HO-1) expression were significantly increased at 12?h after ischemic injury. In contrast, the CoQ10 significantly prevented the upregulation of SOD2 and HO-1 protein expression in ischemic retina. In addition, the CoQ10 significantly blocked activation of astroglial and microglial cells in ischemic retina. Interestingly, the CoQ10 blocked apoptosis by decreasing caspase-3 protein expression in ischemic retina. Bax and phosphorylated Bad (pBad) protein expression were significantly increased in ischemic retina at 12?h. Interestingly, while CoQ10 significantly decreased Bax protein expression in ischemic retina, CoQ10 showed greater increase of pBad protein expression. Of interest, ischemic injury significantly increased mitochondrial transcription factor A (Tfam) protein expression in the retina at 12?h, however, CoQ10 significantly preserved Tfam protein expression in ischemic retina. Interestingly, there were no differences in mitochondrial DNA content among control- or CoQ10-treated groups. Our findings demonstrate that CoQ10 protects RGCs against oxidative stress by modulating the Bax/Bad-mediated mitochondrial apoptotic pathway as well as prevents mitochondrial alteration by preserving Tfam protein expression in ischemic retina. Our results suggest that CoQ10 may provide neuroprotection against oxidative stress-mediated mitochondrial alterations in ischemic retinal injury.