Effects of oxygen and carbon content on nitrogen removal capacities in landfill bioreactors and response of microbial dynamics

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• 作者：Weiqing Xu ; Dong Wu ; Jie Wang ; Xinghua Huang…
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：100
• 期：14
• 页码：6427-6434
• 全文大小：738 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614
• 卷排序：100

文摘

In this study, landfill bioreactors were tested to treat the recalcitrant leachate-nitrogen and the impacts of relevant operational parameters on its conversion were comprehensively investigated. We found that the highly diverse microbial community in landfill bioreactors could be substantially affected by increasing biodegradable carbon and oxygen content, which led to the whole system’s intrinsic nitrogen removal capacity increasing from 50 to 70 %, and meanwhile, the contribution of anammox was detected less than 20 %. The sequencing and q-PCR results showed that microbial community in bioreactor was dominated by Proteobacteria (∼35 %) and Acidobacteria (~20 %) during the whole experiment. The abundance of anammox functioning bacteria (Amx) kept at a stable level (−2.5 to −2.2 log (copies/16S rRNA)) and was not statistically correlated to the abundance of anammox bacteria. However, significant linear correlation (p < 0.05) was determined between the abundance of nirS and Proteobacteria; amoA and AOB. Redundancy analysis (RDA) suggested that although oxygen and biodegradable carbon can both impose effects on microbial community structure, only biodegradable carbon content is the determinant in the total nitrogen removal.KeywordsMicrobial community structureNitrogen pathwaysHigh-throughput sequencingLandfill leachate treatment