Enhanced Effect of Fluorescent Whitening Agent on Peroral Infection for Recombinant Baculovirus in the Host Bombyx mori L

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• 作者：Bing Wang ; Jinyan Shang ; Xunli Liu ; Weizheng Cui ; Xiaofeng Wu and Na Zhao
• 刊名：Current Microbiology
• 出版年：2007
• 出版时间：January, 2007
• 年：2007
• 卷：54
• 期：1
• 页码：5-8
• 全文大小：65 KB

文摘

The low efficiency of the oral infectivity of recombinant polyhedrin-negative baculovirus is a major bottleneck in the application of the baculovirus expression system in the silkworm (Bombyx mori L). In this study, the effects of a fluorescent whitening agent on improving the oral infection for the recombinant Bombyx mori nuclear polyhedrosis virus in silkworm larva and their possible mechanism were investigated. The results showed that the peroral infection can be remarkably enhanced by adding VBL into the larval artificial diet. The maximum infection rate reached as high as 90 % with the concentration of VBL (1 % ), which was then considered as optimal. The total protease activity and pH value of the larval intestinal juice were found to be lower when compared to the control, indicating an abnormal physiological change of the larval digestive system by VBL, which, in turn, resulted in improved peroral infection of recombinant virus.