Transcriptional profile of Paracoccidioides spp. in response to itraconazole
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- 作者：Benedito Rodrigues da Silva Neto (43)
Patrícia Fernanda Zambuzzi Carvalho (43)
Alexandre Melo Bail?o (43)
Wellington Santos Martins (44)
Célia Maria de Almeida Soares (43)
Maristela Pereira (43) - 关键词：Paracoccidioides spp. ; Transcriptional response ; Itraconazole ; Ergosterol
- 刊名：BMC Genomics
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：15
- 期：1
- 全文大小：614 KB
- 参考文献：1. Franco M: Host–parasite relationship in paracoccidioidomycosis. / J Clin Microbiol 1987, 25:5-8.
2. Restrepo A, McEwen JG, Casta?eda E: The habitat of Paracoccidiodes brasiliensis : how far from solving the ridle? / Med Mycol 2001, 39:233-41. CrossRef
3. San-Blas G, Ni?o-Vega G, Iturriaga T: Paracoccidioides brasiliensis and paracoccidioidomycosis: molecular approaches to morphogenesis, diagnosis, epidemiology, taxonomy and genetics. / Med Mycol 2002, 40:225-42. CrossRef
4. Coutinho Z, Silva D, Lazera M, Oliveira RM, Sabroza PC, Wanke B: Paracoccidioidomycosis mortality in Brasil 1980-995. / Cad Saúde Pública 2002, 18:1441-454. CrossRef
5. Shikanai-Yasuda MA, Telles FQ, Mendes RP, Colonbo AL, Moretti ML: Guidelines in paracoccidioidomycosis. / Rev Soc Bras Med Trop 2006, 39:297-10. CrossRef
6. Burgess DS, Hastings RW: A comparison of dynamic characteristics of fluconazole, itraconazole, and amphotericin B against Cryptococcus neoformans using time-kill methodology. / Diagnost Microbiol Infect Dis 2000, 38:87-3. CrossRef
7. Lamb DC, Maspahy S, Kelly DE, Manning NJ, Geber A, Bennett JE, Kelly SL: Purification, reconstitution, and inhibition ofcytochrome P-450 sterol, D22-desaturase from the pathogenic fungus Candida glabrata . / Antimicrob Agents Chemother 1999, 43:1725-728.
8. Bammert GF, Fostel JM: Genome-wide expression patterns in Saccharomyces cerevisiae : comparison of drug treatments and genetic alterations affecting biosynthesis of ergosterol. / Antimicrob Agent Chemother 2000, 44:1255-265. CrossRef
9. Diao Y, Zhao R, Deng X, Leng W, Peng J, JIN Q: Transcriptional profiles of Trichophyton rubrum in response to itraconazole. / Med Mycol 2009, 47:237-47. CrossRef
10. Georgiadou SP, Kontoyiannis DP: The impact of azole resistance on aspergillosis guidelines. / Ann N Y Acad Sci 2012, 12721:5-2.
11. Liu TT, Lee REB, Barker KS, Lee RE, Wei L, Homayouni R, Rogers PD: Genome-wide expression profiling of the response to azole, polyene, echinocandin, and pyrimidine antifungal agents in Candida albicans . / Antimicrob Agents Chemother 2005, 49:2226-236. CrossRef
12. Hoehamer CF, Cummings ED, Hilliard GM, Rogers PD: Changes in the proteome of Candida albicans in response to azole, polyene, and echinocandin antifungal agents. / Antimicrob Agents Chemother 2010, 54:1655-664. CrossRef
13. Georgiadou SP, Kontoyiannis DP: The impact of azole resistance on aspergillosis guidelines. / Ann N Y Acad Sci 2012, 1272:15-2. CrossRef
14. Hayes JD, Flanagan JU, Jowsey IR: Glutathione transferases. / Annu Rev Pharmacol Toxicol 2005, 45:51-8. CrossRef
15. De Backer MD, Ilyina T, Ma XJ, Vandoninck S, Luyten WH, Vanden Bossche H: Genomic profiling of the response of Candida albicans to itraconazole treatment using a DNA microarray. / Antimicrob Agents Chemot 2001, 45:1660-670. CrossRef
16. Munkacsi AB, Pentchev PG, Sturley SL: Spreading the wealth: Niemann-Pick type C proteins bind and transport cholesterol. / Cell Metab 2009, 10:3-. CrossRef
17. Breakspear A, Pasquali M, Brozc K, Donga Y, Kistler HC: Npc1 is involved in sterol trafficking in the filamentous fungus Fusarium graminearum . / Fungal Genet Biol 2011, 48:725-30. CrossRef
18. Van Leeuwen MR, Smant W, de Boer W, Dijksterhuis J: Filipin is a reliablein situ marker of ergosterol in the plasma membrane of germinating conidia (spores) of Penicillium discolor and stains intensively at the site of germ tubeformation. / J Microbiol Method 2008, 74:64-3. CrossRef
19. Beh CT, Rine J: A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular sterol-lipid distribution. / J Cell Sci 2004, 117:2983-996. CrossRef
20. Akins RA: An update on antifungal targets and mechanisms of resistance in Candida albicans . / Med Mycol 2005, 43:285-18. CrossRef
21. Vanden Bossche H, Marichal P, Gorrens J, Bellens D, Moereels H, Janssen PAJ: Mutation in cytochrome P450-dependent 14ademethylase results in decreased affinity for azole antifungals. / Biochemic Soc Transact 1990, 18:56-9.
22. Daum G, Lees ND, Bard M, Dickson R: Biochemistry, cell biology and molecular biology of lipids of Saccharomyces cerevisiae . / Yeast 1998, 14:1471-510. CrossRef
23. Santos GD, Ferri PH, Santos SC, Bao SN, Soares CMA, Pereira M: Oenothein B inhibits the expression of Pb FKS1 transcript and induces morphological changes in Paracoccidioides brasiliensis . / Med Mycol 2007, 45:609-18. CrossRef
24. Liu Q, Siloto RMP, Lehner R, Stone SJ, Weselake RJ: Acyl-CoA: diacylglycerol acyltransferase: molecular biology, biochemistry and biotechnology. / Progr Lipid Resear 2012, 51:350-77. CrossRef
25. Munro CA, Winter K, Buchan A, Henry K, Becker JM, Brown AJ, Bulawa CE, Gow NA: Chs1 of Candida albicans is an essentialchitin synthase required for synthesis of the septum and for cell integrity. / Mol Microbiol 2001, 39:1414-426. CrossRef
26. Banks IR, Specht CA, Donlin MJ, Gerik KJ, Levitz SM, Lodge JK: A chitin synthase and its regulator protein are critical for Chitosan production and growth of the fungal pathogen Cryptococcus neoformans . / Eukaryot Cell 2005, 4:1902-912. CrossRef
27. Vanden Bossche H: Biochemical targets for antifungal azole derivatives hypothesis on the mode of action. / Curr Top Med Mycol 1985, 1:313-51. CrossRef
28. Wang W, Ballatori N: Endogenous glutathione conjugates: occurrence and biological functions. / Pharmacol Rev 1998, 50:335-56.
29. Ghannoum MA, Rice LB: Antifungal agents: mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance. / Clin Microbiol Rev 1999, 12:501-17.
30. Lupetti A, Danesi R, Campa M, Del Tacca M, Kelly S: Molecular basis of resistance to azole antifungals. / Trend Mol Med 2002, 8:76-1. CrossRef
31. Edlind T, Smith L, Henry K, Katiyar S, Nickels J: Antifungal activity in Saccharomyces cerevisiae is modulated by calcium signaling. / Mol Microbiol 2002, 46:257-68. CrossRef
32. Da Silva Cruz AH, Brock M, Zambuzzi-Carvalho PF, Santos-Silva LK, Troian RF, Goes AM, Soares CMA, Pereira M: Phosphorylation is the major mechanism regulating isocitrate lyase activity in Paracoccidioides brasiliensis yeast cells. / FEBS J 2011, 278:2318-332. CrossRef
33. De Oliveira KM, Neto BRS, Parente JA, Da Silva RA, Quintino GO, Voltan AR, Mendes-Giannini MJ, Soares CMA, Pereira M: Intermolecular interactions of the malate synthase of Paracoccidioides spp . / BMC Microbiol 2013, 13:107. CrossRef
34. Fava Netto C, Vegas VS, Sciannamea JJ, Guarnieri DB: Antígeno polissacarídeo do Paracoccidioides brasiliensis . Estudo do tempo de cultura do P.brasiliensis necessário ao preparo do antigen. / Rev Inst Med Trop 1969, 11:177-81.
35. Restrepo A, Jimenez BE: Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined culture medium. / J Clin Microbiol 1980, 12:279-81.
36. Hahn RC, Concei??o YTM, Santos NL, Ferreira JF, Hamdan JS: Disseminated paracoccidioidomycosis: correlation between clinical and in vitro resistance to ketoconazole and trimethoprim sulphamethoxazole. / Mycoses 2003, 46:342-47. CrossRef
37. Hahn RC, Hamdan JS: In vitro susceptibilities of Paracoccidioides brasiliensis yeast form to antifungal drugs. / Mycoses 2000, 43:403-07. CrossRef
38. Freshney R: / Culture of Animal Cells: A Manual of Basic Technique. New York: Alan R Liss; 1987. Inc
39. Hubank M, Schatz AG: Identifying differences in mRNA expression by representational difference analysis of cDNA. / Nucleis Acids Res 1994, 22:5640. CrossRef
40. Pastorian K, Havell L 3rd, Byus CV: Optimization of cDNA representational difference analysis for the identification of differentially expressed mRNAs. / Anal Biochem 2000, 283:89-8. CrossRef
41. Dutra V, Nakazato L, Broetto IS, Vainstein MH, Schrank A: Application of representational difference analysis to identify sequence tags expressed by Metarhizium anisopliae during the infection process of the Boophilus microplus cuticle . / Res Microbiol 2004, 155:245-51. CrossRef
42. Huang X, Madan A: CAP3: a DNA sequence assembly program. / Genom Res 1999, 9:868-77. CrossRef
43. Apweiler R, Bairoch A, Wu CH: Protein sequence databases. / Curr Opin Chem Biol 2004, 8:76-0. CrossRef
44. Kanehisa M, Goto S: KEGG: Kyoto encyclopedia of genes and genomes. / Nucleic Acids Res 2000, 28:27-0. CrossRef
45. Kramer MF: High-throughput real-time quantitative reverse transcription PCR. In / Current Protocols in Molecular Biology. Edited by: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. Hoboken NJ: John Wiley and Sons; 2006:1581e1628.
46. Fonseca CA, Jesuino RS, Felipe MS, Cunha DA, Brito WA, Soares CMA: Two-dimensional electrophoresis andcharacterization of antigens from Paracoccidioides brasiliensis . / Microb Infect 2001, 3:535-42. CrossRef
47. Arthington-Skaggs BA, Jradi H, Desai T, Morrison CJ: Quantitation of ergosterol content: novel method for determination of fluconazole susceptibility of Candida albicans . / J Clin Microbiol 1999, 37:3332-337.
48. Momany M: Using microscopy to explore the duplication cycle. In / The Molecular and Cellular Biology of Filamentous Fungi: A Practical Approach. Edited by: Talbot N. Oxford: University Press; 2001. Oxford
49. Andes D, Lepak A, Pitula A, Marchillo K, Clark J: A simple approach forestimating gene expression in Candida albicans directly from a systemic infectionsite. / J Infect Dis 2005, 192:893-00. CrossRef - 作者单位：Benedito Rodrigues da Silva Neto (43)
Patrícia Fernanda Zambuzzi Carvalho (43)
Alexandre Melo Bail?o (43)
Wellington Santos Martins (44)
Célia Maria de Almeida Soares (43)
Maristela Pereira (43)
43. Departamento de Bioquímica e Biologia Molecular, Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, C.P. 131, 74001-970, Goiania, GO, Brazil
44. Instituto de Informática, Universidade Federal de Goiás, Goiania, Goiás, Brazil - ISSN：1471-2164
文摘
Background Itraconazole is currently used to treat paracoccidioidomycosis. The mechanism of action of azoles has been elucidated in some fungi, although little is known regarding its mechanism of action in Paracoccidioides spp. The present work focused on identification of regulated transcripts using representational difference analysis of Paracoccidioides spp. yeast cells treated with itraconazole for 1 and 2?h. Results Paracoccidioides Pb01 genes up-regulated by itraconazole included genes involved in cellular transport, metabolism/energy, transcription, cell rescue, defense and virulence. ERG11, ERG6, ERG3, ERG5 and ERG25 were up-regulated at multiple time points. In vivo infection experiments in mice corroborated the in vitro results. Ergosterol levels and distribution were evaluated in Paracoccidioides Pb18 yeast cells, and the results demonstrate that both factors were changed in the fungus treated with itraconazole. Conclusion To our knowledge, this is the first transcriptional analysis of Paracoccidioides spp. exposed to a triazole drug. Here acetyl seems to be intensively produced from different metabolic pathways to produce ergosterol by the action of ergosterol synthesis related enzymes, which were also affected in other fungi. Among the genes affected, we identified genes in common with other fungi, as well as genes unique to Paracoccidioides Pb01. Those genes could be considered target to new drugs. Voltage-gated Ca2+ alpha subunit (CAV), Tetracycline resistance protein (TETA) and Hemolisyn-iii channel protein (HLYiii) were found only here and a probably involvement with resistence to itraconazole could be investigated in the future. However our findings do not permit inference to current clinical practice.