A cotton fiber-preferential promoter, PGbEXPA2, is regulated by GA and ABA in Arabidopsis

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• 作者：Yang Li ; Lili Tu ; Zhengxiu Ye ; Maojun Wang ; Wenhui Gao…
• 关键词：Cotton fiber ; α ; expansin ; Promoter analysis ; Gibberellins ; Abscisic acid
• 刊名：Plant Cell Reports
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：34
• 期：9
• 页码：1539-1549
• 全文大小：7,369 KB
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• 作者单位：Yang Li (1)
  Lili Tu (1)
  Zhengxiu Ye (1)
  Maojun Wang (1)
  Wenhui Gao (1)
  Xianlong Zhang (1)
  
  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

Key message PGbEXPA2 (Promoter of GbEXPA2 ) was preferentially and strongly expressed during cotton fiber development, and the 461-bp PGbEXPA2 fragment was essential for responding to exogenous GA and ABA in Arabidopsis. Abstract Cotton fibers are highly elongated single-cell, unbranched and non-glandular seed trichomes. Previous studies have reported that the transcript level of GbEXPA2 is significantly up-regulated during fiber cell elongation, suggesting that GbEXPA2 has an important function in fiber development. In this study, the promoter of GbEXPA2 (839?bp) from the DT sub-genome was isolated from Gossypium barbadense 3-9. Consistent with the expression pattern of GbEXPA2, the promoter PGbEXPA2 was able to express GUS to high levels in elongating fibers, but not in the root, stem, or leaf. In Arabidopsis, GUS activity was only found in the rosette leaf trichomes and rosette leaf vascular tissue, indicating that the transcription factors which bind to PGbEXPA2 in the leaf trichomes of transgenic Arabidopsis were similar to those found in cotton fiber. A deletion analysis of PGbEXPA2 revealed that a 461-bp fragment was sufficient to drive GUS expression in cotton fibers and Arabidopsis rosette leaf trichomes. Exogenous phytohormonal treatments on transgenic Arabidopsis with different promoter lengths (P-839, P-705, P-588 and P-461) showed that GUS activity in Arabidopsis trichomes could be strongly up-regulated by GA and, in contrast, down-regulated by ABA.