The Expression of HSPD1, SCUBE3, CXCL14 and Its Relations with the Prognosis in Osteosarcoma

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• 作者：Wenqing Liang ; Chengwei Yang ; Jiahao Peng ; Yu Qian…
• 关键词：HSPD1 ; SCUBE3 ; CXCL14 ; Osteosarcoma ; Prognosis
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：73
• 期：3
• 页码：763-768
• 全文大小：522 KB
• 参考文献：1.Lang, K., Hao, Y., Huang, H., Lin, I., Rogerio, J. W., & Menzin, J. (2014). Treatment patterns among elderly patients with stage IV breast cancer treated with HER-2-targeted therapy. Journal of Comparative Effectiveness Research, 3(5), 481-90.CrossRef PubMed
  2.Tian, Z., Guo, B., Yu, M., Wang, C., Zhang, H., Liang, Q., et al. (2014). Upregulation of micro-ribonucleic acid-128 cooperating with downregulation of PTEN confers metastatic potential and unfavorable prognosis in patients with primary osteosarcoma. OncoTargets and Therapy, 7, 1601-608.PubMedCentral PubMed
  3.Rappa, F., Farina, F., Zummo, G., David, S., Campanella, C., Carini, F., et al. (2012). HSP-molecular chaperones in cancer biogenesis and tumor therapy: An overview. Anticancer Research, 32(12), 5139-150.PubMed
  4.Lai, C. H., Park, K. S., Lee, D. H., Alberobello, A. T., Raffeld, M., Pierobon, M., et al. (2014). HSP-90 inhibitor ganetespib is synergistic with doxorubicin in small cell lung cancer. Oncogene, 33(40), 4867-876.PubMedCentral CrossRef PubMed
  5.Tanegashima, K., Tsuji, K., Suzuki, K., Shigenaga, A., Otaka, A., & Hara, T. (2013). Dimeric peptides of the C-terminal region of CXCL14 function as CXCL12 inhibitors. FEBS Letters, 587(23), 3770-775.CrossRef PubMed
  6.Grzegrzolka, J., Kurnol, K., Piotrow, P., Pula, B., Kobierzycki, C., Piotrowska, A., et al. (2012). Hsp-27 expression in invasive ductal breast carcinoma. Folia histochemica et cytobiologica/Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, 50(4), 527-33.CrossRef PubMed
  7.Nishizawa, S., Hirohashi, Y., Torigoe, T., Takahashi, A., Tamura, Y., Mori, T., et al. (2012). HSP DNAJB8 controls tumor-initiating ability in renal cancer stem-like cells. Cancer Research, 72(11), 2844-854.CrossRef PubMed
  8.Kubo, T., Shimose, S., Fujimori, J., Furuta, T., Arihiro, K., & Ochi, M. (2015). Does expression of glucose transporter protein-1 relate to prognosis and angiogenesis in osteosarcoma? Clinical Orthopaedics and Related Research, 473, 305-10.CrossRef PubMed
  9.Zhang, C., Yao, C., Li, H., Wang, G., & He, X. (2014). Serum levels of microRNA-133b and microRNA-206 expression predict prognosis in patients with osteosarcoma. International Journal of Clinical and Experimental Pathology, 7(7), 4194-203.PubMedCentral PubMed
  10.Lu, J., Wang, J., Yong, B., Song, G., Zhao, Z., Tang, Q., et al. (2013). Expression of chemokine CXCL14 in primary osteosarcoma and its association with prognosis. Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 33(6), 798-03.PubMed
  11.Gao, F., Hu, X., Xie, X., et al. (2015). Heat shock protein 90 stimulates rat mesenchymal stem cell migration via PI3K/Akt and ERK1/2 pathways. Cell Biochemistry and Biophysics, 71, 481-89.CrossRef PubMed
  12.Healy, E. F., Little, C., & King, P. J. (2014). A model for small heat shock protein inhibition of polyglutamine aggregation. Cell Biochemistry and Biophysics, 69(2), 275-81.CrossRef PubMed
  13.Ohashi, K., Burkart, V., Flohé, S., et al. (2000). Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex. The Journal of Immunology, 164(2), 558-61.CrossRef PubMed
  14.Lu, H. Q., Wang, Y. Z., Sun, P. H., Liang, S. P., Li, J., Wang, X. L., et al. (2012). Effects of heat shock protein 70-2 gene polymorphisms on the transcription of HSP 70-2 mRNA and the translation of HSP 70 protein in lung cancer. Zhonghua yu fang yi xue za zhi (Chinese Journal of Preventive Medicine), 46(5), 452-55.
  15.Xavier, G. M., Panousopoulos, L., & Cobourne, M. T. (2013). Scube3 is expressed in multiple tissues during development but is dispensable for embryonic survival in the mouse. PLoS ONE, 8(1), e55274.PubMedCentral CrossRef PubMed
  16.Chou, C. H., Cheng, Y. F., Siow, T. Y., Kumar, A., Peck, K., & Chang, C. (2013). SCUBE3 regulation of early lung cancer angiogenesis and metastatic progression. Clinical & Experimental Metastasis, 30(6), 741-52.CrossRef
  17.Wu, Y. Y., Peck, K., Chang, Y. L., Pan, S. H., Cheng, Y. F., Lin, J. C., et al. (2011). SCUBE3 is an endogenous TGF-beta receptor ligand and regulates the epithelial-mesenchymal transition in lung cancer. Oncogene, 30(34), 3682-693.CrossRef PubMed
  18.Gu, X. L., Ou, Z. L., Lin, F. J., Yang, X. L., Luo, J. M., Shen, Z. Z., et al. (2012). Expression of CXCL14 and its anticancer role in breast cancer. Breast Cancer Research and Treatment, 135(3), 725-35.CrossRef PubMed
  19.Zeng, J., Yang, X., Cheng, L., Liu, R., Lei, Y., Dong, D., et al. (2013). Chemokine CXCL14 is associated with prognosis in patients with colorectal carcinoma after curative resection. Journal of Translational Medicine, 11, 6.PubMedCentral CrossRef PubMed
  20.Park, C. R., You, D. J., Kim, D. K., Moon, M. J., Lee, C., Oh, S. H., et al. (2013). CXCL14 enhances proliferation and migration of NCI-H460 human lung cancer cells overexpressing the glycoproteins containing heparan sulfate or
• 作者单位：Wenqing Liang (1)
  Chengwei Yang (2)
  Jiahao Peng (3)
  Yu Qian (1)
  Zirui Wang (4)
  
  1. Department of Orthopaedics, Shaoxing People’s Hospital, Shaoxing Hospital of Zhejiang University, 568 Zhongxing North Road, Shaoxing, 312000, Zhejiang Province, People’s Republic of China
  2. Department of Spinal Surgery, Lanzhou General Hospital, Lanzhou Military Area Command, 333 Nanbinhe Road, Lanzhou, 730050, People’s Republic of China
  3. Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang, 050017, People’s Republic of China
  4. East Medical Area, Internal Medicine Ward, Linyi People’s Hospital, 27#Jiefang Road, Linyi City, 276000, Shandong Province, People’s Republic of China
  
• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Biotechnology; Cell Biology; Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1559-0283

文摘

The aim of the study was to explore the expression of three genes, HSPD1, SCUBE3, and CXCL14, in osteosarcoma cells and tissue, as well as their association with the prognosis of patients with osteosarcoma. The expression of HSPD1, SCUBE3, and CXCL14 in osteosarcoma cells was detected by using Western blotting method. siRNA was used to knockdown the expression of the three genes. CCK8 cell proliferation assay was used to observe the effect of siRNA interference on U2OS cell proliferation. The expression of the three genes in osteosarcoma tissue was detected employing immunohistochemical method. Kaplan–Meier survival analysis was used to compare the relations between the expression of the three genes and prognosis. The Western blotting results showed that the expression of Hsp70, SCUBE3 protein, and CXCL14 chemotactic factor in osteosarcoma cells was significantly higher than that in normal osteocytes (p < 0.05). After the three genes were interfered by siRNA, the mRNA and protein expression levels of these genes in osteosarcoma cells were significantly decreased (p < 0.05). The growth rate of U2OS cell after the siRNA interference was significantly lower than that before interference and that in the control group transfected with negative control siRNA (p < 0.05). The result of immunohistochemistry demonstrated that the expression of Hsp70, SCUBE3 protein, and CXCL14 chemotactic factor in osteosarcoma tissues was significantly higher than that in adjacent muscle tissue (p < 0.05). Kaplan–Meier survival analysis indicated that the survival rate of the patients with high expression of those three kinds of genes was obviously lower than that of other patients (p < 0.05). There was no significant difference between the survival rates of patients with high or low expression of two genes (p > 0.05). The expression of HSPD1, SCUBE3, and CXCL14 was all high in osteosarcoma tissues and cells; moreover, the three kinds of genes had close correlations with the prognosis of the patients. Targeted inhibition of these three genes could inhibit the proliferation of the tumor, which may become a new therapeutic target. Keywords HSPD1 SCUBE3 CXCL14 Osteosarcoma Prognosis