A dopamine sensor based on a carbon paste electrode modified with DNA-doped poly(3,4-ethylenedioxythiophene)
详细信息 查看全文
- 作者：Guiyun Xu ; Wenting Wang ; Beibei Li ; Zhonglin Luo ; Xiliang Luo
- 关键词：Conducting polymer ; Poly (3 ; 4 ; ethylenedioxythiophene) ; DNA ; Guanine ; Dopamine
- 刊名：Microchimica Acta
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：182
- 期：3-4
- 页码：679-685
- 全文大小：898 KB
- 参考文献：1. Zhou ZX, Du Y, Dong SJ (2011) Double-strand DNA-templated formation of copper nanoparticles as fluorescent probe for label-free aptamer sensor. Anal Chem 83:5122-127 CrossRef
2. Lin XQ, Kang GF, Lu LP (2007) DNA/Poly(p-aminobenzensulfonic acid) composite bi-layer modified glassy carbon electrode for determination of dopamine and uric acid under coexistence of ascorbic acid. Bioelectrochemistry 70:235-44 CrossRef
3. Liua XG, Penga YH, Qua XJ, Aia XY, Hana RX, Zhub XB (2011) Multi-walled carbon nanotube-chitosan/poly(amidoamine)/DNA nanocomposite modified gold electrode for determination of dopamine and uric acid under coexistence of ascorbic acid. J Electroanal Chem 654:72-8 CrossRef
4. Castillo G, Lamberti I, Mosiello B, Hianik T (2012) Impedimetric DNA aptasensor for sensitive detection of ochratoxin a in food. Electroanalysis 24:512-20 CrossRef
5. LaBean TH, Li H (2007) Constructing novel materials with DNA. Nano Today 2:26-5 CrossRef
6. Tang HW, Duan XR, Feng XL, Liu LB, Wang S, Li YL, Zhu DB (2009) Fluorescent DNA–poly(phenylenevinylene) hybrid hydrogels for monitoring drug release. Chem Commun 6:64-43
7. Sun W, Zhang YY, Hu AH, Lu YX, Shi F, Lei BX, Sun ZF (2013) Electrochemical DNA biosensor based on partially reduced graphene oxide modified carbon ionic liquid electrode for the detection of transgenic soybean A2704-12 gene sequence. Electroanalysis 25:1417-424 CrossRef
8. Luo SCY, Xie H, Chen NY, Yu HH (2009) Trinity DNA detection platform by ultrasmooth and functionalized PEDOT biointerfaces. ACS Appl Mater Interfaces 1:1414-419 CrossRef
9. Bhuvaneswari KN, David WE, Cosmin L, Jadranka TS (2012) A highly sensitive label-free gene sensor based on a single conducting polymer nanowire. Biosens Bioelectron 35:258-64 CrossRef
10. Murat A (2013) A review study of (bio)sensor systems based on conducting polymers. Mater Sci Eng C 33:1853-859 CrossRef
11. Cheng XR, Hau Ben YH, Endo T, Kerman K (2014) Au nanoparticle-modified DNA sensor based on simultaneous electrochemical impedance spectroscopy and localized surface plasmon resonance. Bilsensors Bioelectrons 53:513-18 CrossRef
12. Wilson J, Radhakrishnan H, Sumathi C, Dharuman V (2012) Polypyrrole-polyaniline–Au (PPy–PANi–Au) nano composite films for label-free electrochemical DNA sensing. Sensors Actuators B Chem 171-72:216-22 CrossRef
13. Ferreira VC, Melato AI, Silva AF, Abrantes LM (2011) Conducting polymers with attached platinum nanoparticles towards the development of DNA biosensors. Electrochem Commun 13:993-96 CrossRef
14. Malhotra BD, Chaubey A, Singh SP (2006) Prospects of conducting polymers in biosensors. Anal Chim Acta 578:59-4 CrossRef
15. Prabhakar N, Arora K, Singh SP, Singh H, Malhotra BD (2007) DNA entrapped polypyrrole–polyvinyl sulfonate film for application to electrochemical biosensor. Anal Biochem 366:71-9 CrossRef
16. Jiang XH, Lin XQ (2005) Immobilization of DNA on carbon fiber microelectrodes by using overoxidized polypyrrole template for selective detection of dopamine and epinephrine in the presence of high concentrations of ascorbic acid and uric acid. Analyst 130:391-96
文摘
An electrochemical dopamine sensor was developed by electrochemical modification of a carbon paste electrode with a biocomposite made from poly(3,4-ethylenedioxythiophene) (PEDOT) that was doped with double stranded or single stranded DNA. The resulting conducting biocomposite displays excellent electrocatalytic activity towards the oxidation of dopamine (DA) which is attributed to the presence of the DNA dopant. Under optimum conditions and at a working potential of 300?mV, the modified electrode is capable of detecting DA in the 0.25 to 66?μM concentration range. The detection limit is 74 nM. The sensor is not interfered by uric acid and ascorbic acid. Graphical Abstract PEDOT biocomposites doped with dsDNA (left) and ssDNA (right) can be electrochemically prepared and used for the sensitive and selective detection of dopamine owing to their excellent catalytic activity towards dopamine oxidation.