Wnt1 Participates in Inflammation Induced by Lipopolysaccharide Through Upregulating Scavenger Receptor A and NF-kB

详细信息    查看全文

• 作者：Wenting Zhao ; Zewei Sun ; Shuai Wang ; Zhenwei Li ; Liangrong Zheng
• 关键词：LPS ; inflammatory factors ; wnt1 ; NF ; kB ; scavenger receptor A (SRA)
• 刊名：Inflammation
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：38
• 期：4
• 页码：1700-1706
• 全文大小：1,334 KB
• 参考文献：1.de Winther, M.P.J., K.W. van Dijk, L.M. Havekes, and M.H. Hofker. 2000. Macrophage scavenger receptor class A: a multifunctional receptor in atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology 20: 290-97.PubMed View Article
  2.Chen, Y., F. Wermeling, J. Sundqvist, A. Jonsson, K. Tryggvason, T. Pikkarainen, and M.C.I. Karlsson. 2010. A regulatory role for macrophage class A scavenger receptors in TLR4-mediated LPS responses. European Journal of Immunology 40: 1451-460.PubMed View Article
  3.Yi, H., X. Yu, P. Gao, Y. Wang, S.H. Baek, X. Chen, H.L. Kim, J.R. Subjeck, and X.Y. Wang. 2009. Pattern recognition scavenger receptor SRA/CD204 down-regulates Toll-like receptor 4 signaling-dependent CD8 T-cell activation. Blood 113(23): 5819-828.
  4.Xu, W.Y., L. Wang, H. Ming, Y.Q. Wang, Y.F. Liang, T.T. Zhao, and Y.Z. Wu. 2007. Scavenger receptor A (SR-A) is required for LPS-induced TLR4 mediated NF- κ B activation in macrophages. Molecular Immunology 44: 2315-323.PubMed View Article
  5.Maiese, K., Y.C. Shang, Z.Z. Chong, and J. Hou. 2010. Diabetes mellitus: channeling care through cellular discovery. Current Neurovascular Research 7: 59-4.PubMed Central PubMed View Article
  6.Maiese, K., S.D. Morhan, and Z.Z. Chong. 2007. Oxidative stress biology and cell injury during type 1 and type 2 diabetes mellitus. Current Neurovascular Research 4: 63-1.PubMed Central PubMed View Article
  7.Chong, Z.Z., F. Li, and K. Maiese. 2005. Oxidative stress in the brain: novel cellular targets that govern survival during neurodegenerative disease. Progress in Neurobiology 75: 207-46.PubMed View Article
  8.Bournat, J.C., A.M. Brown, and A.P. Soler. 2000. wnt1 dependent activation of the survival factor NF-kappaB in PC12 cells. Journal of Neuroscience Research 61: 21-2.PubMed View Article
  9.Su, G.L. 2002. Lipopolysaccharides in liver injury: molecular mechanisms of Kupffer cell activation. American Journal of Physiology Gastrointestinal and Liver Physiology 283: G256-5.PubMed View Article
  10.Murphy, J.E., P.R. Tedbury, S. Homer-Vanniasinkam, J.H. Walker, and S. Ponnambalam. 2005. Biochemistry and cell biology of mammalian scavenger receptors. Atherosclerosis 182: 1-5.PubMed View Article
  11.Xu, W., L. Wang, H. Wang, Y. Wang, Y. Liang, T. Zhao, and Y. Wu. 2007. TLR2 and TLR4 agonists synergistically up-regulate SR-A in RAW264.7 through p38. Molecular Immunology 44: 2315-323.PubMed View Article
  12.Amiel, E., A. Alonso, S. Uematsu, S. Akira, M.E. Poynter, and B. Berwin. 2009. Pivotal Advance: Toll-like receptor regulation of scavenger receptor-A-mediated phagocytosis. Journal of Leukocyte Biology 85: 595-05.PubMed Central PubMed View Article
  13.Rimerman, R.A. 2000. wnt1 and MEK1 cooperate to promote cyclin D1 accumulation and cellular transformation. Journal of Biological Chemistry 275: 14736-4742.PubMed View Article
  14.Howe, L.R., H.C. Crawford, K. Subbaramaiah, J.A. Hassell, A.J. Dannenberg, and A.M.C. Brown. 2001. PEA3 is up-regulated in response to wnt1 and activates the expression of cyclooxygenase-2. Journal of Biological Chemistry 276: 20108-0115.PubMed View Article
  15.Yan, D., D. Avtanski, N.K. Saxena, and D. Sharma. 2012. Leptin-induced epithelial-mesenchymal transition in breast cancer cells requires -catenin activation via Akt/GSK3- and MTA1/wnt1 protein-dependent pathways. Journal of Biological Chemistry 287: 8598-612.PubMed Central PubMed View Article
  16.Blavier, L., A. Lazaryev, F. Dorey, G.M. Shackleford, and Y.A. De Clerck. 2006. Matrix metalloproteinases play an active role in wnt1-induced mammary tumorigenesis. Cancer Research 66: 2691-.PubMed View Article
  
• 作者单位：Wenting Zhao (1)
  Zewei Sun (1)
  Shuai Wang (1)
  Zhenwei Li (1)
  Liangrong Zheng (1)
  
  1. Department of Cardiology, First Affiliated Hospital, College of Medicine, Zhejiang University, 79Qingchun Road, Hangzhou, 310003, China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Rheumatology
  Internal Medicine
  Pharmacology and Toxicology
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-2576

文摘

The study investigated the role of wnt1 in the inflammatory response initiated by lipolysaccharide (LPS), and analyzed the association between wnt1, NF-KB, and inflammatory factors. THP-1 cells were activated with phorbol-12-myristate-13-acetate (PMA) and treated with LPS to induce inflammation. THP-1 cells were transfected with wnt1siRNA and overexpression plasmid to explore the relationship among wnt1, SRA, and NF-KB. Inhibitor of β-catenin and siRNA of FZD1were used to investigate the signaling events involved in SRA activation induced by wnt1. Levels of NF-kB protein and inflammatory cytokines were assessed followingwnt1 siRNA and LPS treatment. PMA activation and LPS treatment of THP-1 cells increased wnt1 protein levels. Wnt1 promoted SRA expression through activation of canonical wnt pathway. Wnt1 increased NF-kB protein levels and enhanced the secretion of IL-6, TNF-α, and iNOS through binding to SRA. These findings suggest that wnt1 increased SRA and NF-kB protein levels and participated in the inflammatory response.