Rapid Change of Microbiota Diversity in the Gut but Not the Hepatopancreas During Gonadal Development of the New Shrimp Model Neocaridina denticulata

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• 作者：Man Kit Cheung ; Ho Yin Yip ; Wenyan Nong ; Patrick Tik Wan Law…
• 关键词：Crustacean ; Shrimp ; Hepatopancreas ; Gut ; Ion Torrent ; Gonad development ; Microbiota ; Evolution
• 刊名：Marine Biotechnology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：17
• 期：6
• 页码：811-819
• 全文大小：1,637 KB
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• 作者单位：Man Kit Cheung (1)
  Ho Yin Yip (2)
  Wenyan Nong (2)
  Patrick Tik Wan Law (1) (4)
  Ka Hou Chu (3)
  Hoi Shan Kwan (1)
  Jerome Ho Lam Hui (2)
  
  1. School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
  2. Simon F.S. Li Marine Science Laboratory, School of Life Sciences, Centre for Soybean Research, Partner State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
  4. The Nethersole School of Nursing, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
  3. Simon F.S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Earth sciences
  Oceanography
  
• 出版者：Springer New York
• ISSN：1436-2236

文摘

During evolution of animals, their co-evolution with bacteria has generally been ignored. Recent studies have provided evidences that the symbiotic bacteria in the animal gut can either be essential or contributing to the plasticity of the host. The Crustacea includes crab, crayfish, lobster, and shrimp and represents the second largest subphylum on the planet. Although there are already studies investigating the intestinal bacterial communities in crustaceans, none of them has examined the microbiota in different parts of the digestive system during the gonad development of the host. Here, we utilized a new shrimp model Neocaridina denticulata and sequenced the 16S rRNA using the Ion Torrent platform to survey the bacterial populations colonizing the hepatopancreas, foregut, and intestine, including midgut and hindgut, of the early, mid, and late ovarian maturation stages of the shrimp. The predominant bacteria phylum was found to be Proteobacteria, with more than 80 % reads from the gut flora at the early gonad development belonged to a Coxiella-type bacterium. Distinct bacterial communities can be detected between the hepatopancreas and gut, although no significant difference could be revealed between the different regions of the gut investigated. Surprisingly, during the gonad development, bacterial diversity changed rapidly in the gut but not the hepatopancreas. This study provides the first evidence that microbiota modified differentially in specific regions of the digestive tract during gonadal development of crustaceans. Keywords Crustacean Shrimp Hepatopancreas Gut Ion Torrent Gonad development Microbiota Evolution