The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice

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• 作者：Suyan Duan ; Yingyi Wu ; Chuanyan Zhao ; Mingyu Chen…
• 关键词：Rhein ; db/db mice ; Diabetic nephropathy ; wnt/β ; catenin signaling pathway
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：411
• 期：1-2
• 页码：73-82
• 全文大小：3,428 KB
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• 作者单位：Suyan Duan (1)
  Yingyi Wu (2)
  Chuanyan Zhao (1)
  Mingyu Chen (1)
  Yanggang Yuan (1)
  Changying Xing (1)
  Bo Zhang (1)
  
  1. Department of Nephrology, The First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital), Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China
  2. Department of Rheumatology, The Wuxi 4th People’s Hospital (The Affiliated Hospital of Jiangnan University), 200 Huihe Road, Wuxi, 214062, Jiangsu, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Medical Biochemistry
  Oncology
  Cardiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4919

文摘

The present study aimed to investigate the relationship between wnt/β-catenin signaling pathway and kidney impairment in diabetic nephropathy (DN) mice as well as the renoprotective effect of rhein (RH). Mice were randomly divided into four groups (n = 6): db/db mice treated with RH (DN + RH), db/db mice (DN), db/m mice treated with RH (NC + RH) and db/m mice (NC). RH-treated groups were administered orally at a daily dose 120 mg/kg. Mice were sacrificed after 12 weeks of treatments. In our study, increased albuminuria, together with weight gain and hyperglycemia was observed in the beginning of the study and continued to increase throughout the length of the study (12 weeks). Histopathologic changes were observed in the DN group. Expectedly, mice receiving the treatment with RH were protected from this injury. Meanwhile, the expression of nephrin, a podocyte-specific marker, was significantly reduced while wnt1, p-GSK-3β/tGSK-3β, p-β-catenin/tβ-catenin were higher in the DN group mice when analyzed by immunofluorescence and Western blotting. RH reversed these above changes. wnt/β-catenin signaling pathway participates in RH ameliorating kidney injury in DN mice. The manipulation of RH might act as a promising therapeutic intervention for DN. Keywords Rhein db/db mice Diabetic nephropathy wnt/β-catenin signaling pathway