Anticancer effect of Lycium barbarum polysaccharides on colon cancer cells involves G0/G1 phase arrest

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• 作者：Fang Mao (1)
  Bingxiu Xiao (1)
  Zhen Jiang (1)
  Junwei Zhao (1)
  Xia Huang (1)
  Junming Guo (1)
  
• 关键词：Colon cancer ; Cell cycle ; Cell growth ; Lycium barbarum polysaccharides
• 刊名：Medical Oncology
• 出版年：2011
• 出版时间：March 2011
• 年：2011
• 卷：28
• 期：1
• 页码：121-126
• 全文大小：372KB
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• 作者单位：Fang Mao (1)
  Bingxiu Xiao (1)
  Zhen Jiang (1)
  Junwei Zhao (1)
  Xia Huang (1)
  Junming Guo (1)
  
  1. Ningbo University School of Medicine, 315211, Ningbo, China
  

文摘

Colorectal cancer is one of the most common cancers worldwide. The anticancer effect of Wolfberry (Lycium barbarum) polysaccharide (LBP) on colon cancer cells is largely unknown. To investigate the growth effect of LBP on human colon cancer cell and its possible mechanisms, human colon cancer SW480 and Caco-2 cells were treated with 100-,000?mg/l LBP for 1-?days. Cell growth was measured by MTT assay and crystal violet assay. Distribution of the cell cycle was analyzed by flow cytometry. Western blotting was used to indicate changes in the level of cyclins and cyclin-dependent kinases (CDKs). LBP treatment inhibited both colon cancer cell lines in a dose-dependent manner. At concentrations from 400 to 1,000?mg/l, LBP significantly inhibited the growth of SW480 cells (400?mg/l, P?<?0.01; 800 and 1,000?mg/l, P?<?0.001); while at concentrations from 200 to 1,000?mg/l, LBP significantly inhibited the growth of Caco-2 cells (200?mg/l, P?<?0.05; 400-,000?mg/l, P?<?0.001). Crystal violet assay showed that LBP had a long-term anti-proliferative effect. More importantly, cells were arrested at the G0/G1 phase. The changes in cell-cycle-associated protein, cyclins, and CDKs were consistent with the changes in cell-cycle distribution. This is one of the first studies to focus on LBP-induced interruption of the cell cycle in human colon carcinoma cells. The results suggest that LBP is a candidate anticancer agent.