Modulation of microglia by Wolfberry on the survival of retinal ganglion cells in a rat ocular hypertension model

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• 作者：Kin Chiu (1) (2) (3)
  Hiu-Chi Chan (2)
  Sze-Chun Yeung (1)
  Wai-Hung Yuen (4)
  Sze-Yong Zee (5)
  Raymond Chuen-Chung Chang (1) (2) (3)
  Kwok-Fai So (1) (2) (3) (6)
  
• 关键词：Wolfberry ; Glaucoma ; Microglia ; Neuroprotection
• 刊名：Journal of Ocular Biology, Diseases, and Informatics
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：2
• 期：2
• 页码：47-56
• 全文大小：446KB
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• 作者单位：Kin Chiu (1) (2) (3)
  Hiu-Chi Chan (2)
  Sze-Chun Yeung (1)
  Wai-Hung Yuen (4)
  Sze-Yong Zee (5)
  Raymond Chuen-Chung Chang (1) (2) (3)
  Kwok-Fai So (1) (2) (3) (6)
  
  1. Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
  2. State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
  3. Research Centre of Heart, Brain, Hormone and Healthy Aging, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
  4. Department of Chemistry, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
  5. United College, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China
  6. Laboratory Block, Faculty of Medicine Building, Department of Anatomy, The University of Hong Kong, Rm. L1-49, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China
  

文摘

The active component of Wolfberry (Lycium barbarum), lycium barbarum polysaccharides (LBP), has been shown to be neuroprotective to retinal ganglion cells (RGCs) against ocular hypertension (OH). Aiming to study whether this neuroprotection is mediated via modulating immune cells in the retina, we used multiphoton confocal microscopy to investigate morphological changes of microglia in whole-mounted retinas. Retinas under OH displayed slightly activated microglia. One to 100?mg/kg LBP exerted the best neuroprotection and elicited moderately activated microglia in the inner retina with ramified appearance but thicker and focally enlarged processes. Intravitreous injection of lipopolysaccharide decreased the survival of RGCs at 4?weeks, and the activated microglia exhibited amoeboid appearance as fully activated phenotype. When activation of microglia was attenuated by intravitreous injection of macrophage/microglia inhibitory factor, protective effect of 10?mg/kg LBP was attenuated. The results implicated that neuroprotective effects of LBP were partly due to modulating the activation of microglia.