Molecularly imprinted solid-phase extraction monolithic capillary column for selective extraction and sensitive determination of safranine T in wolfberry

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• 作者：Zihao Su (1)
  Haiyun Zhai (1)
  Zuanguang Chen (2)
  Qing Zhou (1)
  Jiangmei Li (1)
  Zhenping Liu (1)
  
• 关键词：Safranine T ; MIPs ; SPE ; In situ polymerization ; Monolithic capillary column ; Wolfberry
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：406
• 期：5
• 页码：1551-1556
• 全文大小：274 KB
• 作者单位：Zihao Su (1)
  Haiyun Zhai (1)
  Zuanguang Chen (2)
  Qing Zhou (1)
  Jiangmei Li (1)
  Zhenping Liu (1)
  
  1. College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China
  2. School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou, 510006, China
  
• ISSN：1618-2650

文摘

A method was developed to sensitively determine safranine T in wolfberry by molecularly imprinted solid-phase extraction (MISPE) coupled with high-performance liquid chromatography and laser-induced fluorescence detection (HPLC-LIF). The MISPE capillary monolithic column was prepared by water-bath in situ polymerization, using safranine T, methacrylic acid (MAA), and ethylene dimethacrylate (EDMA) as template, functional monomer, and cross-linker, respectively. The properties of the homemade MISPE capillary monolithic column, including capacity and specificity, were investigated under optimized conditions and the morphologies of inner polymers were characterized by scanning electron microscopy (SEM). The mean recoveries of safranine T in wolfberry ranged from 91.2?% to 92.9?% and the intraday and interday relative standard deviation (RSD) values all ranged from 3.4?% to 4.2?%. Good linearity was obtained over 0.001-.0?μg?mL- (r--.9999) with a detection limit (S/N--) of 0.4?ng?g-. Under the selected conditions, enrichment factors of over 90-fold were obtained and the extraction on the monolithic column effectively cleaned up the wolfberry matrix. The results demonstrated that the proposed MISPE-HPLC-LIF method could be applied to sensitively determine safranine T in wolfberry. Figure SEM images of the monolithic column prepared with different initiation reaction methods: a UV initiation; b water bath; c 5000-folds magnification of b; d 20000-folds magnification of b