Loss of aquaporin 3 protein expression constitutes an independent prognostic factor for progression-free survival: an immunohistochemical study on stage pT1 urothelial bladder cancer

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• 作者：Wolfgang Otto (1)
  Peter C Rubenwolf (2)
  Maximilian Burger (3)
  Hans-Martin Fritsche (1)
  Wolfgang R??ler (1)
  Matthias May (4)
  Arndt Hartmann (5)
  Ferdinand Hofst?dter (6)
  Wolf F Wieland (1)
  Stefan Denzinger (1)
  
• 关键词：Urothelial bladder carcinoma ; Stage pT1 ; Aquaporin 3 protein ; Immunohistochemistry ; Progression
• 刊名：BMC Cancer
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：12
• 期：1
• 全文大小：500KB
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  31. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/12/459/prepub
  
• 作者单位：Wolfgang Otto (1)
  Peter C Rubenwolf (2)
  Maximilian Burger (3)
  Hans-Martin Fritsche (1)
  Wolfgang R??ler (1)
  Matthias May (4)
  Arndt Hartmann (5)
  Ferdinand Hofst?dter (6)
  Wolf F Wieland (1)
  Stefan Denzinger (1)
  
  1. Department of Urology of Regensburg University, St. Josef Medical Centre, Regensburg, Germany
  2. Mainz University Medical Center, Department of Urology, Johannes Gutenberg University, Mainz, Germany
  3. Department of Urology, University of Würzburg, Würzburg, Germany
  4. Department of Urology, Klinikum St. Elisabeth, Straubing, Germany
  5. Institute of Pathology, University of Erlangen, Erlangen, Germany
  6. Institute of Pathology, University of Regensburg, Regensburg, Germany
  
• ISSN：1471-2407

文摘

Background Treatment of patients with stage pT1 urothelial bladder cancer (UBC) continues to be a challenge due to its unpredictable clinical course. Reliable molecular markers that help to determine appropriate individual treatment are still lacking. Loss of aquaporin (AQP) 3 protein expression has previously been shown in muscle-invasive UBC. The aim of the present study was to investigate the prognostic value of AQP3 protein expression with regard to the prognosis of stage pT1 UBC. Method AQP 3 protein expression was investigated by immunohistochemistry in specimens of 87 stage T1 UBC patients, who were diagnosed by transurethral resection of the bladder (TURB) and subsequent second resection at a high-volume urological centre between 2002 and 2009. Patients underwent adjuvant instillation therapy with Bacillus Calmette-Guérin (BCG). Loss of AQP3 protein expression was defined as complete absence of the protein within the whole tumour. Expression status was correlated retrospectively with clinicopathological and follow-up data (median: 31 months). Multivariate Cox regression analysis was used to assess the value of AQP3 tumour expression with regard to recurrence-free (RFS), progression-free (PFS) and cancer-specific survival (CSS). RFS, PFS and CSS were calculated by Kaplan-Meier analysis and Log rank test. Results 59% of patients were shown to exhibit AQP3-positive tumours, whereas 41% of tumours did not express the marker. Loss of AQP3 protein expression was associated with a statistically significantly worse PFS (20% vs. 72%, p=0.020). This finding was confirmed by multivariate Cox regression analysis (HR 7.58, CI 1.29 -44.68; p=0.025). Conclusions Loss of AQP3 protein expression in pT1 UBC appears to play a key role in disease progression and is associated with worse PFS. Considering its potential prognostic value, assessment of AQP3 protein expression could be used to help stratify the behavior of patients with pT1 UBC.