Preparation of progenin III from total steroidal saponins of Dioscorea nipponica Makino using a crude enzyme from Aspergillus oryzae strain

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• 作者：Tingqiang Liu (1) (2)
  Hongshan Yu (2)
  Chunying Liu (2)
  Yongming Bao (3)
  Xiangchun Hu (2)
  Yuanhao Wang (2)
  Bing Liu (2)
  Yaoyao Fu (2)
  Sihui Tang (2)
  Fengxie Jin (2)
  
• 关键词：Dioscorea nipponica Makino ; Progenin III ; Enzymatic preparation ; Aspergillus oryzae ; Steroidal saponins
• 刊名：Journal of Industrial Microbiology and Biotechnology
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：40
• 期：5
• 页码：427-436
• 全文大小：513KB
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• 作者单位：Tingqiang Liu (1) (2)
  Hongshan Yu (2)
  Chunying Liu (2)
  Yongming Bao (3)
  Xiangchun Hu (2)
  Yuanhao Wang (2)
  Bing Liu (2)
  Yaoyao Fu (2)
  Sihui Tang (2)
  Fengxie Jin (2)
  
  1. College of Science, Yanbian University, Yanji, 133002, People’s Republic of China
  2. College of Biotechnology, Dalian Polytechnic University, Dalian, 116034, People’s Republic of China
  3. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People’s Republic of China
  
• ISSN：1476-5535

文摘

Progenin III, one of the most active spirostanol saponins, is a potential candidate for anti-cancer therapy due to its strong antitumor activity and low hemolytic activity. However, the concentration of progenin III is extremely low in natural Dioscorea plants. In this paper, the progenin III production from total steroidal saponins of Dioscorea nipponica Makino was studied using the crude enzyme from Aspergillus oryzae DLFCC-38. The crude enzyme converting total steroidal saponins into progenin III was obtained from the A. oryzae DLFCC-38 culture. For enzyme production, the strain was cultured for 72?h at 30?°C with shaking at 150?rpm in 5?% (w/v) malt extract medium containing 2?% (v/v) extract of D. nipponica as the enzyme inducer. The crude enzyme converted total steroidal saponins into major progenin III with a high yield when the reaction was carried out for 9?h at 50?°C and pH 5.0 with the 20?mg/ml of substrate. In the preparation of progenin III, 117?g of crude progenin III was obtained from 160?g of substrate, and the crude product was purified with silica gel column to obtain 60.3?g progenin III of 93.4?% purity.