Streptosporangium shengliensis sp. nov., a novel actinomycete isolated from a lake sediment

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• 作者：Xinhui Zhang (1)
  Chongxi Liu (1)
  Yuejing Zhang (1)
  Haiyan Wang (2)
  Shurui Wang (1)
  Chuang Li (1)
  Xiangjing Wang (2)
  Wensheng Xiang (1)
  
• 关键词：Streptosporangium shengliensis sp. nov. ; Polyphasic taxonomy ; 16S rRNA gene
• 刊名：Antonie van Leeuwenhoek
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：105
• 期：1
• 页码：237-243
• 全文大小：342 KB
• 作者单位：Xinhui Zhang (1)
  Chongxi Liu (1)
  Yuejing Zhang (1)
  Haiyan Wang (2)
  Shurui Wang (1)
  Chuang Li (1)
  Xiangjing Wang (2)
  Wensheng Xiang (1)
  
  1. School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China
  2. Key Laboratory of Microbial Drug Engineering of Heilongjiang Provincial Education Committee, Harbin, 150030, People’s Republic of China
  
• ISSN：1572-9699

文摘

A novel actinomycete, designated strain NEAU-GH7T, was isolated from a lake sediment and characterized using a polyphasic approach. Strain NEAU-GH7T was Gram-stain positive, aerobic, non-spore-forming and produced spherical sporangia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-GH7T formed a monophyletic clade with the closest relative Streptosporangium longisporum DSM 43180T (99.0?%), an association that was supported by a bootstrap value of 74?% in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from its closest relative. Moreover, strain NEAU-GH7T could also be differentiated from S. longisporum DSM 43180T and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0?%) by morphological and physiological characteristics. On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotypic characteristics, strain NEAU-GH7T should be classified as a new species of the genus Streptosporangium, for which the name Streptosporangium shengliensis sp. nov. is proposed. The type strain is NEAU-GH7T (=CGMCC 4.7105T=DSM 45881T).