On the control of Microcystis aeruginosa and Synechococccus species using an algicidal bacterium, Stenotrophomonas F6, and its algicidal compounds cyclo-(Gly-Pro) and hydroquinone

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• 作者：Shengqin Lin ; Mengxin Geng ; Xianglong Liu ; Jing Tan…
• 关键词：Cyanobacterial bloom ; Algicidal bacterium ; Algicidal compounds ; Cyclo ; (Gly ; Pro) ; Hydroquinone
• 刊名：Journal of Applied Phycology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：28
• 期：1
• 页码：345-355
• 全文大小：436 KB
• 参考文献：Anderson DM (1997) Turning back the harmful red tide. Nature 388:513–514CrossRef
  Chen YW, Qin BQ, Teubner K, Dokulil MT (2003) Long-term dynamics of phytoplankton assemblages: Microcystis-domination in Lake Taihu, a large shallow lake in China. J Plankton Res 25:445–453CrossRef
  Chen WM, Sheu FS, Sheu SY (2011) Novel L-amino acid oxidase with algicidal activity against toxic cyanobacterium Microcystis aeruginosa synthesized by a bacterium Aquimarina sp. Enzyme Microb Technol 49:372–379CrossRef PubMed
  Churro C, Alverca E, Sam-Bento F, Paulino S, Figueira VC, Bento AJ, Prabhakar S, Lobo AM, Calado AJ, Pereira P (2009) Effects of bacillamide and newly synthesized derivatives on the growth of cyanobacteria and microalgae cultures. J Appl Phycol 21:429–442CrossRef
  Conley DJ, Paerl HW, Howarth RW, Boesch DF, Seitzinger SP, Havens KE, Lancelot C, Likens GE (2009) Ecology. Controlling eutrophication: nitrogen and phosphorus. Science 323:1014–1015CrossRef PubMed
  Dakhama A, Noie J, Lavoie MC (1993) Isolation and identification of antialgal substances produced by Pseudomonas aeruginosa. J Appl Phycol 5:297–306CrossRef
  DeCaprio AP (1999) The toxicology of hydroquinone—relevance to occupational and environmental exposure. Crit Rev Toxicol 29:283–330CrossRef PubMed
  Doucette GJ, Powell CL (1998) Algal-bacterial interactions: can they determine the PSP-related toxicity of dinoflagellates? In: Reguera B, Blanco J, Fernandez ML, Wyatt T (eds) Harmful algae. Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO: pp 406–409
  Dziga D, Suda M, Bialczyk J, Czaja-Prokop U, Lechowski Z (2007) The alteration of Microcystis aeruginosa biomass and dissolved microcystin-LR concentration following exposure to plant-producing phenols. Environ Toxicol 22:341–346CrossRef PubMed
  Feng Y, Chang X, Zhao L, Li X, Li W, Jiang Y (2012) Nanaomycin A methyl ester, an actinomycete metabolite: algicidal activity and the physiological response of Microcystis aeruginosa. Ecol Eng 51:306–312
  Fraleigh PC, Burnham JC (1988) Myxococcal predation on cyanobacterial populations: nutrient effects. Limnol Oceanogr 33:476–483CrossRef
  Gustafsson S, Hultberg M, Figueroa RI, Rengefors K (2009) On the control of HAB species using low biosurfactant concentrations. Harmful Algae 8:857–863CrossRef
  Houston DR, Synstad B, Eijsink VGH, Stark MJR, Eggleston IM, Aalten DMF (2004) Structure-based exploration of cyclic dipeptide chitinase inhibitors. J Med Chem 47:5713–5720CrossRef PubMed
  Huang TL, Cong HB (2007) A new method for determination of chlorophylls in freshwater algae. Environ Monit Assess 129:1–7CrossRef PubMed
  Imai I, Kimura S (2008) Resistance of the fish-killing dinoflagellate Cochlodinium polykrikoides against algicidal bacteria isolated from the coastal sea of Japan. Harmful Algae 7:360–367CrossRef
  Imamura N, Motoike I, Noda M, Adachi K, Konno A, Fukami H (2000) Argimicin A, a novel anti-cyanobacterial compound produced by an algae-lysing bacterium. J Antibiot 53:1317–1319CrossRef PubMed
  Jeong H, Yim JH, Lee C, Choi SH, Park YK, Yoon SH, Hur CG, Kang HY, Kim D, Lee HH, Park KH, Park SH, Park HS, Lee HK, Oh TK, Kim JF (2005) Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent. Nucl Acids Res 33:7066–7073CrossRef PubMedCentral PubMed
  Kodani S, Imoto A, Mitsutani A, Murakami M (2002) Isolation and identification of the antialgal compound, harmane (1-methyl-β-carboline), produced by the algicidal bacterium, Pseudomonas sp. K44-1. J Appl Phycol 14:109–114CrossRef
  Lee SO, Kato J, Takiguchi N, Kuroda A, Ikeda T, Mitsutani A, Ohtake H (2000) Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28. Appl Environ Microbiol 66:4334–4339CrossRef PubMedCentral PubMed
  Li FM, Hu HY (2005) Isolation and characterization of a novel antialgal allelochemical from Phragmites communis. Appl Environ Microbiol 71:6545–6553CrossRef PubMedCentral PubMed
  Li Z, Geng M, Yang H (2014a) Algicidal activity of Bacillus sp. Lzh-5 and its algicidal compounds against Microcystis aeruginosa. Appl Microbiol Biotechnol 99:981–990CrossRef PubMed
  Li Z, Lin S, Liu X, Tan J, Pan J, Yang H (2014b) A freshwater bacterial strain, Shewanella sp. Lzh-2, isolated from Lake Taihu and its two algicidal active substances, hexahydropyrrolo[1,2-a]pyrazine-1,4-dione and 2, 3-indolinedione. Appl Microbiol Biotechnol 98:4737–4748CrossRef PubMed
  Liu J, Yang Y, Gao J, Jiang J (2011) Comprehensive evaluation method of cyanobacteria bloom hazard in Lake Taihu. J Lake Sci 23:334–338, in Chinese, with English abstractCrossRef
  Long RA, Qureshi A, Faulkner DJ, Azam F (2003) 2-n-Pentyl-4-quinolinol produced by a marine Alteromonas sp. and its potential ecological and biogeochemical roles. Appl Environ Microbiol 69:568–576CrossRef PubMedCentral PubMed
  Mayali X, Azam F (2004) Algicidal bacteria in the sea and their impact on algal blooms. J Eukaryot Microbiol 51:139–144CrossRef PubMed
  Mayali X, Doucette GJ (2002) Microbial community interactions and population dynamics of an algicidal bacterium active against Karenia brevis (Dinophyceae). Harmful Algae 1:277–293CrossRef
  Nakai S, Inoue Y, Hosomi M (2001) Algal growth inhibition effects and inducement modes by plant-producing phenols. Water Res 35:1855–1859CrossRef PubMed
  Nakashima T, Miyazaki Y, Matsuyama Y, Muraoka W, Yamaguchi K, Oda T (2006) Producing mechanism of an algicidal compound against red tide phytoplankton in a marine bacterium gamma-proteobacterium. Appl Microbiol Biotechnol 73:684–690CrossRef PubMed
  Paerl HW, Xu H, McCarthy MJ, Zhu G, Qin B, Li Y, Gardner WS (2011) Controlling harmful cyanobacterial blooms in a hyper-eutrophic lake (Lake Taihu, China): the need for a dual nutrient (N & P) management strategy. Water Res 45:1973–1983CrossRef PubMed
  Pápista É, Ács É, Böddi B (2002) Chlorophyll-a determination with ethanol—a critical test. Hydrobiologia 485:191–198CrossRef
  Park JS, Jung MY (2012) Development of high-performance liquid chromatography–time-of-flight mass spectrometry for the simultaneous characterization and quantitative analysis of gingerol-related compounds in ginger products. J Agr Food Chem 60:10015–10026CrossRef
  Pokrzywinski KL, Place AR, Warner ME, Coyne KJ (2012) Investigation of the algicidal exudate produced by Shewanella sp. IRI-160 and its effect on dinoflagellates. Harmful Algae 19:23–29CrossRef
  Qin B, Xu P, Wu Q, Luo L, Zhang Y (2007) Environmental issues of Lake Taihu, China. Hydrobiologia 581:3–14CrossRef
  Sakata T, Yoshikawa T, Nishitarumizu S (2011) Algicidal activity and identification of an algicidal substance produced by marine Pseudomonas sp. C55a-2. Fisheries Sci 77:397–402CrossRef
  Sengco MR (2009) Prevention and control of Karenia brevis blooms. Harmful Algae 8:623–628CrossRef
  Shao J, Li R, Lepo JE, Gu JD (2013) Potential for control of harmful cyanobacterial blooms using biologically derived substances: problems and prospects. J Environ Manag 125:149–155CrossRef
  Sigee DC, Glenn R, Andrews MJ, Bellinger EG, Butler RD, Epton HAS, Hendry RD (1999) Biological control of cyanobacteria: principles and possibilities. Hydrobiologia 395/396:161–172CrossRef
  Skerratt JH, Bowman JP, Hallegraeff G, James S, Nichols PD (2002) Algicidal bacteria associated with blooms of a toxic dinoflagellate in a temperate Australian estuary. Mar Ecol Prog Ser 244:1–15
  Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bact Rev 35:171–205PubMedCentral PubMed
  Su J, Yang X, Zheng T, Hong H (2007a) An efficient method to obtain axenic cultures of Alexandrium tamarense—a PSP-producing dinoflagellate. J Microbiol Meth 69:425–430CrossRef
  Su JQ, Yang XR, Zheng TL, Tian Y, Jiao NZ, Cai LZ, Hong HS (2007b) Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense. Harmful Algae 6:799–810CrossRef
  Tian C, Liu X, Tan J, Lin S, Li D, Yang H (2012) Isolation, identification and characterization of an algicidal bacterium from Lake Taihu and preliminary studies on its algicidal compounds. J Environ Sci (China) 24:1823–1831CrossRef
  Wang X, Gong L, Liang S, Han X, Zhu C, Li Y (2005) Algicidal activity of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa. Harmful Algae 4:433–443CrossRef
  Wang B, Yang X, Lu J, Zhou Y, Su J, Tian Y, Zhang J, Wang G, Zheng T (2012) A marine bacterium producing protein with algicidal activity against Alexandrium tamarense. Harmful Algae 13:83–88CrossRef
  Wu X, Xi W, Ye W, Yang H (2007) Bacterial community composition of a shallow hypertrophic freshwater lake in China, revealed by 16S rRNA gene sequences. FEMS Microbiol Ecol 61:85–96CrossRef PubMed
  Xie P (2008) Historical development of cyanobacteria with bloom disaster in lake Taihu. Science Press, Beijing, in Chinese
  Yamamoto Y, Suzuki K (1990) Distribution and algal‐lysing activity of fruiting Myxobacteria in lake Suwa. J Phycol 26:457–462CrossRef
  Yamamoto Y, Kouchiwa T, Hodoki Y, Hotta K, Uchida H, Harada KI (1998) Distribution and identification of actinomycetes lysing cyanobacteria in a eutrophic lake. J Appl Phycol 10:391–397CrossRef
  Yang C, Zhou J, Liu S, Fan P, Wang W, Xia C (2013a) Allelochemical induces growth and photosynthesis inhibition, oxidative damage in marine diatom Phaeodactylum tricornutum. J Exp Mar Biol Ecol 444:16–23CrossRef
  Yang F, Wei HY, Li XQ, Li YH, Li XB, Yin LH, Pu YP (2013b) Isolation and characterization of an algicidal bacterium indigenous to lake Taihu with a red pigment able to lyse Microcystis aeruginosa. Biomed Environ Sci 26:148–154PubMed
  Ye W, Tan J, Liu X, Lin S, Pan J, Li D, Yang H (2011) Temporal variability of cyanobacterial populations in the water and sediment samples of Lake Taihu as determined by DGGE and real-time PCR. Harmful Algae 10:472–479CrossRef
  Yoshikawa K, Adachi K, Nishijima M, Takadera T, Tamaki S, Harada K, Mochida K, Sano H (2000) Beta-cyanoalanine production by marine bacteria on cyanide-free medium and its specific inhibitory activity toward cyanobacteria. Appl Environ Microbiol 66:718–722CrossRef PubMedCentral PubMed
  Zheng X, Zhang B, Zhang J, Huang L, Lin J, Li X, Zhou Y, Wang H, Yang X, Su J, Tian Y, Zheng T (2012) A marine algicidal actinomycete and its active substance against the harmful algal bloom species Phaeocystis globosa. Appl Microbiol Biotechnol 97:9207–9215CrossRef PubMed
  Zhu J-S, Halpern GM, Hjones K (1998) The scientific rediscovery of an ancient Chinese herbal medicine Cordyceps sinensis: part I. J Alt Complem Med 4:289–303CrossRef
  
• 作者单位：Shengqin Lin (1)
  Mengxin Geng (1)
  Xianglong Liu (1)
  Jing Tan (1)
  Hong Yang (1)
  
  1. State Key Laboratory of Microbial metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, People’s Republic of China
  
• 刊物主题：Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology;
• 出版者：Springer Netherlands
• ISSN：1573-5176

文摘

Cyanobacterial blooms occur annually in Lake Taihu, causing large ecological and economic losses. As part of efforts to enhance the strategies explored to control cyanobacterial blooms, an algicidal bacterium, F6, was isolated from Lake Taihu and then identified as a Stenotrophomonas species by 16S rDNA sequence analysis. The results showed that Stenotrophomonas F6 exhibited strong algicidal activity against dominant cyanobacterial bloom-forming species in Lake Taihu, mainly via excretion of extracellular algicidal compounds. The algicidal compounds were subsequently extracted with ethyl acetate, purified by high-performance liquid chromatography (HPLC) and identified as cyclo-(Gly-Pro) and hydroquinone by gas chromatography–mass spectrometry (GC–MS) and 1H nuclear magnetic resonance (NMR) analysis. Cyclo-(Gly-Pro) exerted a strong algicidal effect against Microcystis aeruginosa 9110 with a 50 % effective concentration (EC_50,24h) value of 5.9 mg L−1, but had no algicidal effect on Synechococcus sp. BN60. Hydroquinone showed stronger algicidal activity against both M. aeruginosa 9110 and Synechococcus sp. BN60, with EC_50,24h values of 0.96 and 5.6 mg L−1, respectively. Through characterization of algicidal process, the algicidal activity of Stenotrophomonas F6 was found to be dependent on the concentration of algicidal compounds, and the secreted algicidal compounds could be detected only when the cell density of F6 was above a threshold of 2–4 × 108 CFU mL−1. Taken together, these results suggest that the algicidal bacterium F6 and its two algicidal compounds have the potential for use as bio-agents for controlling cyanobacterial blooms in Lake Taihu. Keywords Cyanobacterial bloom Algicidal bacterium Algicidal compounds Cyclo-(Gly-Pro) Hydroquinone