Diagnosis and phylogenetic analysis of ovine pulmonary adenocarcinoma in China

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• 作者：Keshan Zhang (1)
  Hanjin Kong (2)
  Yongjie Liu (1)
  Youjun Shang (1)
  Bin Wu (2)
  Xiangtao Liu (1)
  
• 关键词：China ; Molecular identification ; Ovine pulmonary adenocarcinoma ; Phylogenetic analysis
• 刊名：Virus Genes
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：48
• 期：1
• 页码：64-73
• 全文大小：1,256 KB
• 作者单位：Keshan Zhang (1)
  Hanjin Kong (2)
  Yongjie Liu (1)
  Youjun Shang (1)
  Bin Wu (2)
  Xiangtao Liu (1)
  
  1. State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping No.1, Yanchangpu, Lanzhou, 730046, Gansu, People鈥檚 Republic of China
  2. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, People鈥檚 Republic of China
  
• ISSN：1572-994X

文摘

Ovine pulmonary adenocarcinoma (OPA) is a lung tumor of sheep caused by jaagsiekte sheep retrovirus (JSRV). OPA is common in sheep, and it is most commonly observed in China. Without preventative vaccines and serological diagnostic tools for assay of OPA, identification of JSRV based on reverse transcription polymerase chain reaction (RT-PCR) is very important for prevention and control measures for OPA in practice management. In this study, the diagnosis of OPA was made from analysis of clinical signs, pathological observations, JSRV-like particle discovery, and RT-PCR of the target env gene. The phylogenetic analysis showed that the China Shandong (SD) strain studied in this article belonged to exogenous JSRV, and it was very similar to 92k3, which was isolated from sheep in the Kenya (Y18305). The current study reported a severe outbreak of OPA in Shandong Province, China. The observations could offer a comparative view of the env gene of JSRV.