Immobilization of Xylanase on Poly (Ethylene Glycol) Methyl Ether 5000 and its Self-Extractive Bioconversion for the Production of Xylo-Oligosaccharides

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• 作者：Xin Li (1)
  Zongxing Shan (1)
  Xiangyang Song (1)
  Jia Ouyang (1)
  Yong Xu (1)
  Qiang Yong (1)
  Shiyuan Yu (1)
  
• 关键词：Endo ; β ; 1 ; 4 ; xylanase ; Immobilization ; PEGylation ; Self ; extractive bioconversion ; Xylo ; oligosaccharides
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：172
• 期：4
• 页码：2022-2029
• 全文大小：286 KB
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• 作者单位：Xin Li (1)
  Zongxing Shan (1)
  Xiangyang Song (1)
  Jia Ouyang (1)
  Yong Xu (1)
  Qiang Yong (1)
  Shiyuan Yu (1)
  
  1. Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, College of Chemical Engineering, Nanjing Forestry University, No. 159 Long Pan Road, Nanjing, 210037, Jiangsu Province, China
  
• ISSN：1559-0291

文摘

Endo-β-1,4-xylanase derived from Trichoderma reesei was covalently immobilized on poly (ethylene glycol) methyl ether 5000 (mPEG5000), and the resulting immobilized enzyme had a residual activity of 72.4?% with 82.9?% of PEGylated amino groups. Compared with the free enzyme, the immobilized xylanase was stable at pH values in the range of 4.0-.0 and temperatures in the range of 50-5?°C. A self-extractive bioconversion system composed of immobilized xylanase, mPEG5000, and sodium citrate was used to produce xylo-oligosaccharides and provided a better distribution of the xylo-oligosaccharides than the free enzyme. Furthermore, the immobilized xylanase could be effectively recovered in situ following the hydrolysis reaction.