Single-cell genomics: An overview

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• 作者：Qichao Wang (1)
  Xianmin Zhu (1) (2)
  Yun Feng (1)
  Zhigang Xue (1)
  Guoping Fan (2) (3) (4)
  
• 关键词：single ; cell genomics ; next ; generation sequencing ; RNA ; seq ; single ; nucleotide variation ; copy ; number variation ; DNA methylation
• 刊名：Frontiers in Biology
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：8
• 期：6
• 页码：569-576
• 全文大小：
• 作者单位：Qichao Wang (1)
  Xianmin Zhu (1) (2)
  Yun Feng (1)
  Zhigang Xue (1)
  Guoping Fan (2) (3) (4)
  
  1. Stem Cell Research Center, Department of Regenerative Medicine, Tongji University School of Medicine, Tongji University, Shanghai, 200092, China
  2. Advanced Institute of Translational Medicine, Tongji University, Shanghai, 200092, China
  3. School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China
  4. Department of Human Genetics, UCLA, Los Angeles, CA, 90095, USA
  
• ISSN：1674-7992

文摘

The newly developed next-generation sequencing platforms, in combination with genome-scale amplification methods, provide a powerful tool to study genomics from a single cell. This mini-review summarizes the technologies of single cell genomics and their applications in several areas of biomedical research including stem cells, cancer biology and reproductive medicine. Particularly, it highlights recent advances in single cell exome sequencing, RNA-seq, and genome sequencing. The application of these powerful techniques will shed new light on the fundamental principles of gene transcription and genome organization at single-cell level and improve our understanding of cellular heterogeneity and diversity in multicellular organisms.