A method suitable for DNA extraction from humus-rich soil

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• 作者：Tianjin Miao (1)
  Song Gao (1)
  Shengwei Jiang (2)
  Guoshi Kan (1)
  Pengju Liu (1)
  Xianming Wu (1)
  Yingfeng An (1)
  Shuo Yao (1)
  
• 关键词：DNA extraction ; Humic substances ; Metagenomic DNA ; Soil DNA ; Soil microbiology
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：36
• 期：11
• 页码：2223-2228
• 全文大小：837 KB
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• 作者单位：Tianjin Miao (1)
  Song Gao (1)
  Shengwei Jiang (2)
  Guoshi Kan (1)
  Pengju Liu (1)
  Xianming Wu (1)
  Yingfeng An (1)
  Shuo Yao (1)
  
  1. College of Biosciences and Biotechnology, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, 110161, People鈥檚 Republic of China
  2. State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, People鈥檚 Republic of China
  
• ISSN：1573-6776

文摘

A rapid and convenient method for extracting DNA from soil is presented. Soil DNA is extracted by direct cell lysis in the presence of EDTA, SDS, phenol, chloroform and isoamyl alcohol (3-methyl-1-butanol) followed by precipitation with 2-propanol. The extracted DNA is purified by modified DNA purification kit and DNA gel extraction kit. With this method, DNA extracted from humus-rich dark brown forest soil was free from humic substances and, therefore, could be used for efficient PCR amplification and restriction digestion. In contrast, DNA sample extracted with the traditional CTAB-based method had lower yield and purity, and no DNA could be extracted from the same soil sample with a commonly-used commercial soil DNA isolation kit. In addition, this method is time-saving and convenient, providing an efficient choice especially for DNA extraction from humus-rich soils.