Nucleotide mutations in purA gene and pur operon promoter discovered in guanosine- and inosine-producing Bacillus subtilis strains

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• 作者：Jiangchao Qian ; Xianpeng Cai ; Ju Chu ; Yingping Zhuang and Siliang Zhang
• 关键词：Bacillus subtilis ; Guanosine ; Inosine ; PurA ; Pur operon
• 刊名：Biotechnology Letters
• 出版年：2006
• 出版时间：June 2006
• 年：2006
• 卷：28
• 期：12
• 页码：937-941
• 全文大小：283 KB

文摘

The promoter region of the pur operon, which contains 12 genes for inosine monophosphate biosynthesis from phosphoribosylpyrophosphate, and the purA gene, encoding the adenylosuccinate synthetase, were compared among wild-type and three purine-producing Bacillus subtilis strains. A single nucleotide deletion at position 55 (relative to translation start site) in purA gene was found in a high inosine-producing strain and in a high guanosine-producing strain, which correlates with the absence of adenylosuccinate synthetase activity in these strains. Within the pur operon promoter of high guanosine-producing strain, in addition to a single nucleotide deletion in PurBox1 and a single nucleotide substitution in PurBox2, there were 4 substitutions in the flanking region of the PurBoxes and 32 nucleotide mutations in the 5′ untranslated region. These mutations may explain the purine accumulation in purine-producing strains and be helpful to the rational design of high-yield recombinant strains.