Global transcriptomic analysis of the response of Corynebacterium glutamicum to ferulic acid

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• 作者：Can Chen ; Junfeng Pan ; Xiaobing Yang ; He Xiao ; Yaoling Zhang…
• 关键词：Corynebacterium glutamicum ; Ferulic acid ; KatA ; SigE ; Lignocellulosic biomass
• 刊名：Archives of Microbiology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：199
• 期：2
• 页码：325-334
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Microbial Ecology; Biochemistry, general; Cell Biology; Biotechnology; Ecology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1432-072X
• 卷排序：199

文摘

Corynebacterium glutamicum can survive by using ferulic acid as the sole carbon source. In this study, we assessed the response of C. glutamicum to ferulic acid stress by means of a global transcriptional response analysis. The transcriptional data showed that several genes involved in degradation of ferulic acid were affected. Moreover, several genes related to the stress response; protein protection or degradation and DNA repair; replication, transcription and translation; and the cell envelope were differentially expressed. Deletion of the katA or sigE gene in C. glutamicum resulted in a decrease in cell viability under ferulic acid stress. These insights will facilitate further engineering of model industrial strains, with enhanced tolerance to ferulic acid to enable easy production of biofuels from lignocellulose.