Biological identification and determination of optimum growth conditions for four species of Navicula

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• 作者：Xiaobo Zhao (1) (2)
  Shaojun Pang (1)
  Feng Liu (1)
  Tifeng Shan (1)
  Jing Li (1) (2)
  
• 关键词：Navicula ; SSU ; rbcL ; culture conditions
• 刊名：Acta Oceanologica Sinica
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：33
• 期：8
• 页码：111-118
• 全文大小：1,001 KB
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• 作者单位：Xiaobo Zhao (1) (2)
  Shaojun Pang (1)
  Feng Liu (1)
  Tifeng Shan (1)
  Jing Li (1) (2)
  
  1. Algal Culture Collection Centre, Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
  2. University of Chinese Academy of Sciences, Beijing, 100049, China
  
• ISSN：1869-1099

文摘

Four species in the genus Navicula were isolated using the serial dilution method. Based on scanning electron microscopy (SEM) and sequence comparisons of two segments of genes (small ribosomal subunit and large subunit of Rubisco), the species were identified as Navicula perminuta, N. pseudacceptata, N. vara, and N. rhynchocephala. Based on phylogenetic analysis and culture trials, there was a close relationship between N. perminuta and N. vara. Growth of these species was evaluated using measurements of optical density at 680 nm (OD680) under various environmental factors. Results showed that the optimum culture conditions were 25°C, 50-00 μmol photons m? s?, pH 8.0, and salinities from 25 to 30. However, the favorable salinity for N. perminuta was surprisingly high at 35. Nutrient requirement analysis demonstrated that growth of Navicula depended on the availability of SiO3 2?/sup>. Their relative growth rates (RGR) peaked at the highest tested level (0.25 mmol/L). The optimal concentrations of NO3 ?/sup> and PO4 3?/sup> were 3.6 mmol/L and 0.18 mmol/L, respectively. Culture of these Navicula species for abalone or sea cucumber aquaculture should take these factors into consideration.