Cell Growth and Shikonin Production of Arnebia euchroma in a Periodically Submerged Airlift Bioreactor

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• 作者：Feng Ge ; Xiaofan Yuan ; Xiaodong Wang ; Bing Zhao and Yuchun Wang
• 关键词：Arnebia euchroma ; bioreactor ; plant cell culture ; shikonin
• 刊名：Biotechnology Letters
• 出版年：2006
• 出版时间：April 2006
• 年：2006
• 卷：28
• 期：8
• 页码：525-529
• 全文大小：211 KB

文摘

Arnebia euchroma was grown in a 2-l periodically submerged, airlift bioreactor (PSAB) in which the non-submerged (immobilization culture) and submerged (suspension culture) operations were controlled automatically. PSAB had advantages in improving cell growth, shikonin content, shikonin production and cell aggregation compared with suspension culture. Under the optimal submerged/non-submerged period of 10 min/15 h, the shikonin content (4.6 % , w/w) and, cell dry mass (16.8 g/l) were 229 and 26 % higher than those in suspension culture.