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作者单位:Xiujuan Chen (1) Yukun Huang (1) Xiaoyuan Ma (1) Fei Jia (1) Xiaofei Guo (1) Zhouping Wang (1)
1. State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China
刊物类别:Chemistry and Materials Science
刊物主题:Chemistry Analytical Chemistry Inorganic Chemistry Physical Chemistry Characterization and Evaluation Materials Monitoring, Environmental Analysis and Environmental Ecotoxicology
出版者:Springer Wien
ISSN:1436-5073
文摘
We are presenting an aptasensor for the sensitive determination of fumonisin B1 (FB-1) via electrochemical impedance spectroscopy (EIS) and applying aptamer-based biorecognition. A thiolated aptamer for FB-1 was anchored onto the surface of gold nanoparticles (AuNPs) on a glassy carbon electrode. A significant increase in resistance (Ret) is found on interaction with FB-1 in the 0.1 nM to 100?μM concentration range, and the detection limit is as low as 2 pM. The assay was applied to determine FB-1 in spiked maize samples and gave recovery rates ranging from 91 to 105?%. The results demonstrate this method to present new possibilities in the application of aptamers in food safety analysis.