Phenotypic and molecular identification of Sporothrix isolates of clinical origin in Northeast China

详细信息    查看全文

• 作者：Xiaohong Yu (1)
  Zhe Wan (2)
  Zhenying Zhang (1)
  Fuqiu Li (3)
  Ruoyu Li (2)
  Xiaoming Liu (1)
  
• 关键词：Sporotrichosis ; Sporothrix globosa ; Calmodulin gene ; Northeast China
• 刊名：Mycopathologia
• 出版年：2013
• 出版时间：2 - August 2013
• 年：2013
• 卷：176
• 期：1
• 页码：67-74
• 全文大小：325KB
• 参考文献：1. Marimon R, Cano J, Gené J, Sutton DA, Kawasaki M, Guarro J. / Sporothrix brasiliensis, / S. globosa, and / S. / mexicana, three new / Sporothrix species of clinical interest. J Clin Microbiol. 2007;45:3198-06. CrossRef
  2. Marimon R, Gené J, Cano J, Guarro J. / Sporothrix luriei: a rare fungus from clinical origin. Med Mycol. 2008;46:621-. CrossRef
  3. de Meyer EM, de Beer ZW, Summerbell RC, Moharram AM, de Hoog GS, Vismer HF, Wingfield MJ. Taxonomy and phylogeny of new wood- and soil-inhabiting / Sporothrix species in the / Ophiostoma / stenoceras-em class="a-plus-plus">Sporothrix schenckii complex. Mycologia. 2008;100:647-1. CrossRef
  4. Madrid H, Cano J, Gené J, Bonifaz A, Toriello C, Guarro J. / Sporothrix globosa, a pathogenic fungus with widespread geographical distribution. Rev Iberoam Micol. 2009;26:218-2. CrossRef
  5. Oliveira MME, Almeida-Paes R, Muniz MM, Barros MBL, Gutierrez-Galhardo MC, Zancopé-Oliveira RM. Sporotrichosis caused by / Sporothrix globosa in Rio de Janeiro, Brazil: case report. Mycopathologia. 2010;169:359-3. CrossRef
  6. Romeo O, Scordino F, Criseo G. New insight into molecular phylogeny and epidemiology of / Sporothrix schenckii species complex based on calmodulin-encoding gene analysis of Italian isolates. Mycopathologia. 2011;172:179-6. CrossRef
  7. Zhang J, Lin J. Clinical analysis of 316 cases of cutaneous sporotrichosis [in Chinese]. Chin J Mycol. 2008;3:207-0.
  8. Song Y, Yao L, Zhong S, Tian Y, Liu Y, Li S. Infant sporotrichosis in northeast China: a report of 15 cases. Int J Dermatol. 2011;50:522-. CrossRef
  9. Li S, Liu H, Zheng H, Chen S, Fu A, Wang H, Song Y, Yao L, Liu Y. Clinical analysis of 585 cases of cutaneous sporotrichosis [in Chinese]. Chin J Dermatol. 2011;44:161-.
  10. Mesa-Arango AC, Del Rocío Reyes-Montes M, Pérez-Mejía A, Navarro-Barranco H, Souza V, Zúniga G, Toriello C. Phenotyping and genotyping of / Sporothrix schenckii isolates according to geographic origin and clinical form of sporotrichosis. J Clin Microbiol. 2002;40:3004-1. CrossRef
  11. Criseo G, Romeo O. Ribosomal DNA sequencing and phylogenetic analysis of environmental / Sporothrix / schenckii strains: comparison with clinical isolates. Mycopathologia. 2010;169:351-. CrossRef
  12. Marimon R, Gené J, Cano J, Trilles L, Dos Santos Lazéra M, Guarro J. Molecular phylogeny of / Sporothrix schenckii. J Clin Microbiol. 2006;44:3251-. CrossRef
  13. O’Donnell K, Nirenberg HI, Aoki T, Cigelnik E. A multigene phylogeny of the / Gibberella fujikuroi species complex: detection of additional phylogenetically distinct species. Mycoscience. 2000;41:61-8. CrossRef
  14. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596-. CrossRef
  15. Ishizaki H, Kawasaki M, Mochizuki T, Jin X, Kagawa S. Environmental isolates of / Sporothrix schenckii in China. Jpn J Med Mycol. 2002;43:257-0. CrossRef
  16. Neyra E, Fonteyne PA, Swinne D, Fauche F, Bustamante B, Nolard N. Epidemiology of human sporotrichosis investigated by amplified fragment length polymorphism. J Clin Microbiol. 2005;43:1348-2. CrossRef
  17. Kwon-Chung KJ. Comparison of isolates of / Sporothrix schenckii obtained from fixed cutaneous lesions with isolates from other types of lesions. J Infect Dis. 1979;139:424-1. CrossRef
  18. Mehta KIS, Sharma NL, Kanga AK, Mahajan VK, Ranjan N. Isolation of / Sporothrix schenckii from the environmental sources of cutaneous sporotrichosis patients in Himachal Pradesh, India: results of a pilot study. Mycoses. 2007;50:496-01. CrossRef
  19. Zhang Z, Liu X, Lv X, Lin J. Variation in genotype and higher virulence of a strain of / Sporothrix schenckii causing disseminated cutaneous sporotrichosis. Mycopathologia. 2011;172:439-6. CrossRef
  
• 作者单位：Xiaohong Yu (1)
  Zhe Wan (2)
  Zhenying Zhang (1)
  Fuqiu Li (3)
  Ruoyu Li (2)
  Xiaoming Liu (1)
  
  1. Department of Dermatology and Venerology, The 1st Affiliated Hospital of Dalian Medical University, No.222, Zhongshan Road, Dalian, 116011, China
  2. Department of Dermatology and Venerology, Centre for Medical Mycology and Mycoses, The First Hospital, Peking University, No.8, Xishiku Street, Xicheng District, Beijing, 100034, China
  3. Department of Dermatology and Venerology, The Second Hospital of Jilin University, No.218, Ziqiang Street, Changchun, 130041, China
  

文摘

Sporotrichosis is the most common deep mycosis in Northeast China which is an area of high epidemicity due to contact with reeds or cornstalks. In this study, we have characterized a total of 74 clinical isolates from fixed cutaneous, lymphocutaneous and disseminated clinical forms and from Heilongjiang, Jilin, and Liaoning provinces, respectively. All isolates (previously as Sporothrix schenckii) were identified as Sporothrix globosa according to their phenotypic characteristics and calmodulin gene sequences analysis. They were subdivided into two sub-clades (S. globosa I and S. globosa II). Most of our isolates (71/74) presented restricted growth at 37?°C, which differed from a previous report. Up to now, S. globosa is the only pathogenic species in Northeast China, no matter what kind of clinical form and which region it is isolated from. Most of our clinical isolates (68/74) were clustered with three Chinese environmental isolates reported in the literature. The new findings of S. globosa isolates on division and thermotolerance at 37?°C described in this study will help us gain a better understanding of S. globosa.