文摘

Pepsin (Pep) accelerated the electron transferring rate of excited 3-aminophathlate and enhanced luminol-dissolved oxygen chemiluminescence (CL) intensity, and the flow injection (FI) luminol-Pep CL system was first developed. It was found that the CL intensity of luminol-Pep reaction could be remarkably inhibited by pseudoephedrine (PE); the decrement of CL intensity was linear to the logarithm of PE concentration in the range of 0.1?00.0?nmol?L? with a detection limit of 0.03?nmol?mL? (3σ). At a flow rate of 2.0?mL?min?, the complete process including washing and sampling was performed within 40?s, offering a sample throughput of 90?h?. This proposed method was successfully applied to determining PE in rat serum for 18?h after intragastric administration with the elimination ratio of 42.34?% and recoveries from 90.3 to 110.6?%. The pharmacokinetic results showed that PE could be rapidly absorbed into serum with peak concentration (C max) of 1.45?±-.18?g?L? at the time (T max) of 1.49?±-.02?h; the absorption half-life (0.35?±-.04?h), elimination half-life (1.86?±-.24?h), the area under curve (109.81?±-.03?mg?L??h?), mean residence time (3.82?±-.27?h), and elimination rate constant (2.26?±-.23?L?g??h?) in rats vivo were derived, respectively. The possible CL mechanism of luminol-Pep-PE reaction was discussed by FI-CL, fluorescence, and molecular docking (MD) methods. Graphical Abstract ?/em>