A third-generation biosensor for hydrogen peroxide based on the immobilization of horseradish peroxidase on a disposable carbon nanotubes modified screen–printed electrode

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• 作者：Shuxia Xu ; Xiaojiao Qin ; Xinfeng Zhang ; Chengxiao Zhang
• 关键词：Disposable biosensor ; Screen printed carbon nanotubes electrode ; Horseradish peroxidase ; Direct electron transfer ; Hydrogen peroxide biosensor
• 刊名：Microchimica Acta
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：182
• 期：7-8
• 页码：1241-1246
• 全文大小：419 KB
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• 作者单位：Shuxia Xu (1) (2)
  Xiaojiao Qin (1)
  Xinfeng Zhang (1)
  Chengxiao Zhang (2)
  
  1. Mineral Resources Chemistry Key Laboratory of Sichuan Higher Education Institutions, College of Material and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu, 610059, China
  2. School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi’an, 710062, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

A screen-printed carbon nanotube?(CNTs) based disposable third generation biosensor for hydrogen peroxide (H₂O₂) was constructed by mixing CNTs with a cellulose acetate binder on an epoxy substrate. The surface was covered with a layer consisting of horseradish peroxidase (HRP) crosslinked to bovine serum albumin with glutaraldehyde. The CNTs acted as both the electrode material and charge promoter to facilitate the direct electron transfer between immobilized HRP and the modified electrode. At a working potential of ?00?mV (vs. Ag/AgCl), the biosensor displays an excellent electrocatalytic response to the reduction of H₂O₂ without the aid of a mediator. It has a fast (3?s) amperometric response and a linear calibration in the concentration range 0.005-.1?mmol?L?, with a 0.85?μM detection limit (at an S/N of 3). The biosensor can be easily produced, is stable and reproducible.