Vesicle formation between single-chained cationic surfactant and plasmid DNA and its application in cell transfection
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  • 作者:Xia Guo (1)
    Fengyan Yu (1)
    Xiangmei Ran (1)
    Xiaojuan Song (1)
    Jiatong Ding (1)
    Ying Wang (1)
  • 关键词:Follicle ; stimulating hormone ; Surfactant ; Plasmid DNA ; Vesicle
  • 刊名:Colloid & Polymer Science
  • 出版年:2014
  • 出版时间:December 2014
  • 年:2014
  • 卷:292
  • 期:12
  • 页码:3103-3111
  • 全文大小:742 KB
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    16. Hsu W-L, Chen H-L, Liou W, Lin H-K, Liu W-L (2005) Mesomorphic complexes of DNA with the mixtures of a cationic surfactant and a neutral lipid. Langmuir 21:9426 Xiaojuan Song (1)
    Jiatong Ding (1)
    Ying Wang (1)

    1. School of Chemistry and Chemical Engineering, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225002, People’s Republic of China
  • ISSN:1435-1536
文摘
In the present study, we found that plasmid DNA could induce single-chained cationic surfactants cetyltrimethylammonium bromide (CTAB), dodecyltrimethylammonium bromide (DTAB), and dodecyltriethyl ammonium bromide (DEAB) to form vesicles once its concentration reached a critical value. Moreover, the gene for follicle-stimulating hormone was delivered into cells with these single-chained cationic surfactant/DNA vesicles and the transfection efficiency was comparable to that with lipofectamine?2000, a famous and widely used commercial transfection reagent, and also to that using electroporation method, although it was generally thought conventional single-chained cationic surfactant was not suitable for gene transfer. The conventional single-chained cationic surfactant is very cheap and stable and the vesicles are very easy to be prepared. Thereby, this study may suggest that the vesicles formed between plasmid DNA and surfactant should be prospective to transfer DNA.

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