Quantitative analysis of promoter methylation of the EDNRB gene in gastric cancer

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• 作者：Kaixiong Tao (1)
  Chuanqing Wu (1)
  Ke Wu (2)
  Wei Li (1)
  Gaoxiong Han (1)
  Xiaoming Shuai (1)
  Guobin Wang (1)
  
• 关键词：Gastric cancer ; EDNRB ; Methylation ; Pyrosequencing
• 刊名：Medical Oncology
• 出版年：2012
• 出版时间：March 2012
• 年：2012
• 卷：29
• 期：1
• 页码：107-112
• 全文大小：395KB
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• 作者单位：Kaixiong Tao (1)
  Chuanqing Wu (1)
  Ke Wu (2)
  Wei Li (1)
  Gaoxiong Han (1)
  Xiaoming Shuai (1)
  Guobin Wang (1)
  
  1. Department of Laparoscopic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, Hubei, 430022, People’s Republic of China
  2. Department of Gastroenterology Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, Hubei, 430022, People’s Republic of China
  

文摘

Hypermethylation has been shown in the promoter region of the endothelin receptor B (EDNRB) gene in several human tumors. However, its role in gastric cancer formation is still unclear. In this study, the methylation status of the EDNRB gene in paired gastric cancer tissues and adjacent normal tissues from 96 patients was detected quantitatively using pyrosequencing. The results showed the methylation of promoter of EDNRB gene in gastric cancer (50.42?±?9.03%) was significantly higher than in adjacent normal tissues (6.47?±?2.98%) (P?<?0.01). Among 96 tumor tissues, promoter hypermethylation of the EDNRB gene was correlated with tumor infiltration (T1: 47.4?±?7.31% T2:48.2?±?9.17% T3:52.9?±?6.48% T4:53.2?±?10.45%), lymph node metastasis (N0:45.4?±?6.99% N1:49.0?±?9.10% N2:52.0?±?8.40% N3:53.7?±?9.92%), and distant metastasis (M0:48.9?±?6.99% M1:53.9?±?11.98%) (P?<?0.05), but it was not associated with other clinicopathological characteristics. In addition, the treatment of the human gastric cancer cell line, SGC-7901, with demethylation agent can restore the expression of EDNRB. Our results suggest that promoter hypermethylation of EDNRB gene is highly prevalent in gastric cancer, which may play a role in the pathogenesis of gastric cancer. Futhermore, hypermethylation of EDNRB gene was remarkably related to infiltration and metastasis of gastric cancer and may attribute to the tumor progression.