Salmonella typhimurium-induced M1 macrophage polarization is dependent on the bacterial O antigen

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• 作者：Fengling Luo ; Xiaoming Sun ; Zhen Qu…
• 关键词：Macrophage polarization ; O antigen ; Salmonella typhimurium
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：32
• 期：2
• 全文大小：888 KB
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• 作者单位：Fengling Luo (1)
  Xiaoming Sun (1)
  Zhen Qu (1)
  Xiaolian Zhang (1)
  
  1. State Key Laboratory of Virology, Medical Research Institute of Wuhan University and Department of Immunology and Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University School of Medicine, Donghu Road 185#, Wuhan, 430071, Hubei Province, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

Recently, macrophages were shown to be capable of differentiating toward two phenotypes after antigen stimulation: a classically activated (M1) or an alternatively activated phenotype (M2). To investigate the effect of Salmonella enteric serovar typhimurium (S. typhimurium) on macrophage differentiation, we compared macrophage phenotypes after infection of murine bone marrow-derived macrophages with wild-type S. typhimurium and its isogenic rfc mutant. S. typhimurium C5 induced M1 macrophage polarization and enhanced inducible nitric oxide synthase expression by macrophages; this induction was dependent on Toll-like receptor 4. In contrast, the Δrfc mutant (S. typhimurium C5 rfc::Kmr) lost this function and induced an M2 response in the macrophages. Here, we propose that S. typhimurium C5 is capable of polarizing macrophages towards the M1 phenotype and that this polarization is dependent on the O antigen encoded by rfc. Our finding indicates that M1 macrophage polarization induced by S. typhimurium may be related to the ability of this intracellular bacterium to survive and replicate within macrophages, which is essential for systemic disease.