Molecular cloning, tissue expression and SNP analysis in the goat nerve growth factor gene

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• 作者：Xiaopeng An (1)
  Long Bai (1)
  Jinxing Hou (1)
  Haibo Zhao (1)
  Jiayin Peng (1)
  Yunxuan Song (1)
  Jiangang Wang (1)
  Binyun Cao (1)
  
• 关键词：NGF gene ; Coding region ; Polymorphism ; Litter size ; Goat
• 刊名：Molecular Biology Reports
• 出版年：2013
• 出版时间：February 2013
• 年：2013
• 卷：40
• 期：2
• 页码：857-863
• 全文大小：564KB
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• 作者单位：Xiaopeng An (1)
  Long Bai (1)
  Jinxing Hou (1)
  Haibo Zhao (1)
  Jiayin Peng (1)
  Yunxuan Song (1)
  Jiangang Wang (1)
  Binyun Cao (1)
  
  1. College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, People’s Republic of China
  
• ISSN：1573-4978

文摘

In this study, we cloned the full coding region of NGF gene from the caprine ovary. Result showed the caprine NGF cDNA (GenBank Accession No. JQ308184) contained a 726?bp open reading frame encoding a protein with 241 amino acid residues. Bioinformatic analysis indicated that caprine NGF amino acid sequence was 83-9?% identical to that of mouse, pig, dog, human and bovine. It was predicted that caprine NGF contained nine serine phosphorylation loci, four threonine phosphorylation loci and nine specific PKC phosphorylation loci. The NGF mRNA expression pattern showed that NGF gene was expressed highly in ovary. This work provided an important experimental basis for further research on the function of NGF in goat. A single nucleotide polymorphism (A705G) in the coding region of NGF gene was detected by PCR–RFLP and DNA sequencing in 630 goats of three breeds. The frequencies of G allele were 0.52-.61, and frequencies of A allele were 0.48-.39 for SN, GZ and BG breeds, respectively. The does with GG genotype had higher litter size than those with GA and AA genotypes (P?<?0.05). Hence, the biochemical and physiological functions, together with the results obtained in our investigation, suggest that the NGF gene could serve as a genetic marker for litter size in goat breeding.