Enhanced Biodegradation of Alkane Hydrocarbons and Crude Oil by Mixed Strains and Bacterial Community Analysis

详细信息    查看全文

• 作者：Yu Chen (1) (2)
  Chen Li (3)
  Zhengxi Zhou (3)
  Jianping Wen (3) (4) (5)
  Xueyi You (1)
  Youzhi Mao (3)
  Chunzhe Lu (3)
  Guangxin Huo (3)
  Xiaoqiang Jia (3) (4) (5)
  
• 关键词：Biodegradation ; Crude oil ; Chromatography ; Microbial growth ; Biosurfactant ; Waste treatment
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：172
• 期：7
• 页码：3433-3447
• 全文大小：
• 参考文献：1. Abboud, M. M., Kheifat, K. M., Batarseh, M., Tarawneh, K. A., Al-Mustafa, A., & Al-Madadhah, M. (2007). Different optimization conditions required for enhancing the biodegradation of linear alkylbenzosulfonate and sodium dodecyl sulfate surfactants by novel consortium of / Acinetobacter calcoaceticus and / Pantoea agglomerans. Enzyme and Microbial Technology, 41(4), 432-39. CrossRef
  2. Adelowo, O. O., Alagbe, S. O., & Ayandele, A. A. (2006). Time-dependent stability of used engine oil degradation by cultures of / Pseudomonas fragi and / Achromobacter aerogenes. African Journal of Biotechnology, 5(24), 2476-479.
  3. Arutchelvi, J., Joseph, C., & Doble, M. (2011). Process optimization for the production of rhamnolipid and formation of biofilm by / Pseudomonas aeruginosa CPCL on polypropylene. / Biochemical Engineering Journal, 56(1-), 37-5. CrossRef
  4. Binazadeh, M., Karimi, I. A., & Li, Z. (2009). Fast biodegradation of long chain / n-alkanes and crude oil at high concentrations with / Rhodococcus sp Moj-3449. / Enzyme and Microbial Technology, 45(3), 195-02. CrossRef
  5. Bodtker, G., Hvidsten, I. V., Barth, T., & Torsvik, T. (2009). Hydrocarbon degradation by / Dietzia sp A14101 isolated from an oil reservoir model column. / Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 96(4), 459-69. CrossRef
  6. Cerqueira, V. S., Hollenbach, E. B., Maboni, F., Vainstein, M. H., Camargo, F. A. O., MdCR, P., & Bento, F. M. (2011). Biodegradation potential of oily sludge by pure and mixed bacterial cultures. / Bioresource Technology, 102(23), 11003-1010. CrossRef
  7. Gojgic-Cvijovic, G. D., Milic, J. S., Solevic, T. M., Beskoski, V. P., Ilic, M. V., Djokic, L. S., Narancic, T. M., & Vrvic, M. M. (2012). Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study. / Biodegradation, 23(1), 1-4. CrossRef
  8. Jadhav, M., Kalme, S., Tamboli, D., & Govindwar, S. (2011). Rhamnolipid from / Pseudomonas desmolyticum NCIM-2112 and its role in the degradation of Brown 3REL. / Journal of Basic Microbiology, 51(4), 385-96. CrossRef
  9. Lal, B., & Khanna, S. (1996). Degradation of crude oil by / Acinetobacter calcoaceticus and / Alcaligenes odorans. The Journal of Applied Bacteriology, 81(4), 355-62.
  10. Leahy, J. G., & Colwell, R. R. (1990). Microbial degradation of hydrocarbons in the environment. / Microbiological Reviews, 54(3), 305-15.
  11. Lotfabad, T. B., Abassi, H., Ahmadkhaniha, R., Roostaazad, R., Masoomi, F., Zahiri, H. S., Ahmadian, G., Vali, H., & Noghabi, K. A. (2010). Structural characterization of a rhamnolipid-type biosurfactant produced by / Pseudomonas aeruginosa MR01: enhancement of di-rhamnolipid proportion using gamma irradiation. / Colloids and Surfaces B-Biointerfaces, 81(2), 397-05. CrossRef
  12. Luckarift, H. R., Sizemore, S. R., Farrington, K. E., Fulmer, P. A., Biffinger, J. C., Nadeau, L. J., & Johnson, G. R. (2011). Biodegradation of medium chain hydrocarbons by / Acinetobacter venetianus 2AW immobilized to hair-based adsorbent mats. / Biotechnology Progress, 27(6), 1580-587. CrossRef
  13. Mehdi, H., & Giti, E. (2008). Investigation of alkane biodegradation using the microtiter plate method and correlation between biofilm formation, biosurfactant production and crude oil biodegradation. / International Biodeterioration & Biodegradation, 62(2), 170-78. CrossRef
  14. Mishra, S., Sarma, P. M., & Lal, B. (2004). Crude oil degradation efficiency of a recombinant / Acinetobacter baumannii strain and its survival in crude oil-contaminated soil microcosm. / Fems Microbiology Letters, 235(2), 323-31. CrossRef
  15. Mohanty, S., & Mukherji, S. (2012). Alteration in cell surface properties of / Burkholderia spp. during surfactant-aided biodegradation of petroleum hydrocarbons. / Applied Microbiology and Biotechnology, 94(1), 193-04. CrossRef
  16. Nayak, A. S., Vijaykumar, M. H., & Karegoudar, T. B. (2009). Characterization of biosurfactant produced by / Pseudoxanthomonas sp PNK-04 and its application in bioremediation. / International Biodeterioration & Biodegradation, 63(1), 73-9. CrossRef
  17. Obayori, U. S., Adebusoye, S. A., Adewale, A. O., Oyetibo, G. O., Oluyemi, O. O., Amokun, R. A., & Ilori, M. O. (2009). Differential degradation of crude oil (Bonny Light) by four / Pseudomonas strains. / Journal of Environmental Sciences China, 21(2), 243-48. CrossRef
  18. Okieimen, C. O., & Okieimen, F. E. (2002). Effect of natural rubber processing sludge on the degradation of crude oil hydrocarbons in soil. / Bioresource Technology, 82(1), 95-7. CrossRef
  19. Ollis, D. (1992). Slick solution for oil spills. / Nature, 358, 453-54. CrossRef
  20. Salam, L. B., Obayori, O. S., Akashoro, O. S., & Okogie, G. O. (2011). Biodegradation of bonny light crude oil by bacteria isolated from contaminated soil. / International Journal of Agriculture and Biology, 13(2), 245-50.
  21. Sei, K., Sugimoto, Y., Mori, K., Maki, H., & Kohno, T. (2003). Monitoring of alkane-degrading bacteria in a sea-water microcosm during crude oil degradation by polymerase chain reaction based on alkane-catabolic genes. / Environmental Microbiology, 5(6), 517-22. CrossRef
  22. Siegmund, I., & Wagner, F. (1991). New method for detecting rhamnolipids excreted by / Pseudomonas species during growth on mineral agar. / Biotechnology Techniques, 5(4), 265-68. CrossRef
  23. Thavasi, R., Jayalakshmi, S., & Banat, I. M. (2011). Application of biosurfactant produced from peanut oil cake by / Lactobacillus delbrueckii in biodegradation of crude oil. / Bioresource Technology, 102(3), 3366-372. CrossRef
  24. Thavasi, R., Jayalakshmi, S., & Banat, I. M. (2011). Effect of biosurfactant and fertilizer on biodegradation of crude oil by marine isolates of / Bacillus megaterium, / Corynebacterium kutscheri and / Pseudomonas aeruginosa. Bioresource Technology, 102(2), 772-78. CrossRef
  25. Vyas, T. K., & Dave, B. P. (2007). Effect of crude oil concentrations, temperature and pH on growth and degradation of crude oil by marine bacteria. / Indian Journal of Marine Sciences, 36(1), 76-5.
  26. Wang, X. B., Chi, C. Q., Nie, Y., Tang, Y. Q., Tan, Y., Wu, G., & Wu, X. L. (2011). Degradation of petroleum hydrocarbons (C6-C40) and crude oil by a novel / Dietzia strain. / Bioresource Technology, 102(17), 7755-761. CrossRef
  27. Wang, Z., & Fingas, M. F. (2003). Development of oil hydrocarbon fingerprinting and identification techniques. / Marine Pollution Bulletin, 47(9), 423-52. CrossRef
  28. Yerima, M. B., Balogun, A. A., Farouq, A. A., & Muhammad, S. (2009). Laboratory based degradation of light crude oil by aquatic phycomycetes. / African Journal of Biotechnology, 8(16), 3851-853.
  
• 作者单位：Yu Chen (1) (2)
  Chen Li (3)
  Zhengxi Zhou (3)
  Jianping Wen (3) (4) (5)
  Xueyi You (1)
  Youzhi Mao (3)
  Chunzhe Lu (3)
  Guangxin Huo (3)
  Xiaoqiang Jia (3) (4) (5)
  
  1. Department of Environmental Science, School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, People’s Republic of China
  2. China Offshore Environmental Services Ltd., Tianjin, 300452, People’s Republic of China
  3. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
  4. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, 300072, People’s Republic of China
  5. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People’s Republic of China
  
• ISSN：1559-0291

文摘

In this study, two strains, Acinetobacter sp. XM-02 and Pseudomonas sp. XM-01, were isolated from soil samples polluted by crude oil at Bohai offshore. The former one could degrade alkane hydrocarbons (crude oil and diesel, 1:4 (v/v)) and crude oil efficiently; the latter one failed to grow on alkane hydrocarbons but could produce rhamnolipid (a biosurfactant) with glycerol as sole carbon source. Compared with pure culture, mixed culture of the two strains showed higher capability in degrading alkane hydrocarbons and crude oil of which degradation rate were increased from 89.35 and 74.32?±-.09 to 97.41 and 87.29?±-.41?%, respectively. In the mixed culture, Acinetobacter sp. XM-02 grew fast with sufficient carbon source and produced intermediates which were subsequently utilized for the growth of Pseudomonas sp. XM-01 and then, rhamnolipid was produced by Pseudomonas sp. XM-01. Till the end of the process, Acinetobacter sp. XM-02 was inhibited by the rapid growth of Pseudomonas sp. XM-01. In addition, alkane hydrocarbon degradation rate of the mixed culture increased by 8.06 to 97.41?% compared with 87.29?% of the pure culture. The surface tension of medium dropping from 73.2?×-0? to 28.6?×-0??N/m. Based on newly found cooperation between the degrader and the coworking strain, rational investigations and optimal strategies to alkane hydrocarbons biodegradation were utilized for enhancing crude oil biodegradation.