Down-regulation of the expression of CCAAT/enhancer binding protein α gene in cervical squamous cell carcinoma

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• 作者：Zemin Pan ; Weinan Zheng ; Jinli Zhang ; Rui Gao ; Dongmei Li ; Xiaoqing Guo…
• 关键词：C/EBPα gene ; Gene expression ; Cervical squamous cell carcinoma
• 刊名：BMC Cancer
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：593 KB
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• 刊物主题：Cancer Research; Oncology; Stem Cells; Animal Models; Internal Medicine;
• 出版者：BioMed Central
• ISSN：1471-2407

文摘

Background Cervical carcinoma is the second most common cancer and is an important cause of death in women worldwide. CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular differentiation and proliferation in a variety of tissues. However, the role of C/EBPα gene in cervical cancer is still not clear. Methods We investigated the expression of C/EBPα gene in cervical squamous cell carcinoma. C/EBPα mRNA level was measured by real-time quantitative RT-PCR in cervical cancer tissues and their adjacent normal tissues. C/EBPα protein level was measured by immunohistochemistry. Methylation in the promoter of C/EBPα gene was detected by MALDI TOF MassARRAY. We transfected HeLa cells with C/EBPα expression vector. C/EBPα expression in HeLa cells was examined and HeLa cell proliferation was measured by MTT assay and HeLa cells migration was analyzed by matrigel-coated transwell migration assays. Results There were significant difference in C/EBPα protein expression between chronic cervicitis and cervical carcinoma (P--.001). CEBPα mRNA level was significantly lower in cervical cancer tissues than in normal cervical tissues (P--.01). Methylation of the promoter of CEBPα gene in CpG 5, CpG-14.15, CpG-19.20 were significantly higher in cervical cancer than in normal cervical tissues (P--.05, P--.01, P--.05, respectively). CEBPα pcDNA3.1 construct transfected into HeLa cells inhibited cell proliferation and decreased cell migration. Conclusions Our results indicate that reduced C/EBPα gene expression may play a role in the development of cervical squamous cell carcinoma.