RETRACTED ARTICLE: Inhibition of Lipopolysaccharide (LPS)-Induced Inflammatory Responses by Selenium in Bovine Mammary Epithelial Cells in Primary Culture
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- 作者：Zhengkai Wei ; Minjun Yao ; Yimeng Li ; Zhengtao Yang ; Xiaosheng Feng
- 关键词：selenium ; bovine mammary epithelial cell ; LPS
- 刊名：Inflammation
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：38
- 期：1
- 页码：152-158
- 全文大小：1,747 KB
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文摘
Selenium, in the form of selenoproteins, plays a pivotal role in anti-inflammatory processes and antioxidant defense system. The aim of this study was to examine the effects of selenium on lipopolysaccharide (LPS)-induced inflammatory responses in bovine mammary epithelial cells (bMEC) and to investigate the potential mechanism. bMEC viability was measured by MTT assay. TNF-α, IL-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) messenger RNA (mRNA) expressions were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The activation of nuclear factor-kappa B (NF-κB) was determined by Western blotting. The results showed that the mRNA expressions of these inflammatory factors were significantly inhibited by selenium in a dose-dependent manner. At protein levels, Western blot analysis demonstrated that selenium dose-dependently decreased NF-κB p65 translocating from the cytoplasm to the nucleus. Taken together, these results suggest that the anti-inflammatory property of selenium in LPS-stimulated primary bMEC may be attributed to the downregulation of NF-κB activation.