A rapid determination of drug candidate tyrosol galactoside in rat plasma by HPLC and its application to the pharmacokinetics study

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• 作者：Qingwei Wang (1)
  Yu Wang (2)
  Yating Deng (3)
  Tianrao Shi (2)
  Xueying Liu (2)
  Xiaoli Sun (2)
  Xiaoye Li (2)
  Dan Zhou (2)
  
• 关键词：Tyrosol galactoside ; Pharmacokinetics ; Bioavailability ; Glycosides
• 刊名：European Journal of Drug Metabolism and Pharmacokinetics
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：35
• 期：3-4
• 页码：131-136
• 全文大小：257KB
• 参考文献：1. Ahn EY, Jiang Y, Zhang Y et al (2008) Cytotoxicity of / p-tyrosol and its derivatives may correlate with the inhibition of DNA replication initiation. Oncol Rep 19:527-34
  2. Chernyshov GA, Plotnikov MB, Smol’iakova VI, Krasnov EA (2007) Therapeutic effect of / p-tyrosol on myocardial electric instability induced by coronary occlusion. Eksp Klin Farmakol 70:23-5
  3. Dinnella C, Minichino P, D’Andrea AM, Monteleone E (2007) Bioaccessibility and antioxidant activity stability of phenolic compounds from extra-virgin olive oils during in vitro digestion. J Agric Food Chem 55:8423-429 CrossRef
  4. Panossian A, Nikoyan N, Ohanyan N et al (2008) Comparative study of Rhodiola preparations on behavioral despair of rats. Phytomedicine 15:84-1 CrossRef
  5. Panossian A, Wikman G, Kaur P, Asea A (2009) Adaptogens exert a stress-protective effect by modulation of expression of molecular chaperones. Phytomedicine 16:617-22 CrossRef
  6. Puel C, Mardon J, Agalias A et al (2008) Major phenolic compounds in olive oil modulate bone loss in an ovariectomy/inflammation experimental model. J Agric Food Chem 56:9417-422 CrossRef
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• 作者单位：Qingwei Wang (1)
  Yu Wang (2)
  Yating Deng (3)
  Tianrao Shi (2)
  Xueying Liu (2)
  Xiaoli Sun (2)
  Xiaoye Li (2)
  Dan Zhou (2)
  
  1. Department of Pharmacy, The Second Affiliated Hospital, Fourth Military Medical University, 17 Changlexi Street, Xi’an, 710032, People’s Republic of China
  2. Department of Medicinal Chemistry, Fourth Military Medical University, 17 Changlexi Street, Xi’an, 710032, People’s Republic of China
  3. Department of Pharmacology, Fourth Military Medical University, Xi’an, 710032, People’s Republic of China
  

文摘

A simple and sensitive HPLC method has been developed and validated for the determination of tyrosol galactoside (TG) in rat plasma. After one-step protein precipitation with methanol, plasma samples were separated on an Ultimate AQ–C18 column (150?mm?×?4.6?mm, 5?μm) using acetonitrile–water (7:93, v/v) as mobile phase at a flow rate of 1.2?mL/min. The ultraviolet detection wavelength was set at 275?nm. The lower limit of quantification was 1.140?μg/mL. The calibration curve was linear over a concentration range of 1.140-28.0?μg/mL. The assay accuracy and precision were within the range of 99.6-03.0 and 2.17-.23%, respectively. The developed method was successfully applied to the pharmacokinetics study of TG in rats after intravenous and oral administration. The bioavailability of TG in rats is 27.9%.