Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae

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• 作者：Liu Yang ; Ce Shi ; Xiaoying Mu ; Chao Liu ; Ke Shi ; Wenjiao Zhu…
• 关键词：P450 ; Tobacco ; Solanum torvum ; StoCYP77A2 ; Transformation ; Verticillium wilt
• 刊名：Plant Biotechnology Reports
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：9
• 期：4
• 页码：167-177
• 全文大小：3,665 KB
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• 作者单位：Liu Yang (1) (2)
  Ce Shi (1)
  Xiaoying Mu (1)
  Chao Liu (1)
  Ke Shi (1)
  Wenjiao Zhu (1)
  Qing Yang (1)
  
  1. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People鈥檚 Republic of China
  2. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People鈥檚 Republic of China
  
• 刊物主题：Plant Sciences; Cell Biology; Plant Biochemistry; Biotechnology; Agriculture;
• 出版者：Springer Japan
• ISSN：1863-5474

文摘

Plant P450s play an important role in plant response to environmental stimuli, especially to pathogen challenge, besides leading to the biosynthesis of secondary metabolites. In this work, a Verticillium dahliae-induced cDNA sequence was cloned from wild eggplant Solanum torvum and designated StoCYP77A2. StoCYP77A2 encodes a 511-amino acid peptide that shows sequence homology to the plant CYP77 family. StoCYP77A2 is expressed in a tissue-specific manner with the highest expression level in stem. The expression level of StoCYP77A2 was increased in wild eggplants which were infected with Verticillium dahlia. We expressed StoCYP77A2 in V. dahliae-sensitive tobacco, and the transgenic tobacco plants were more resistant to V. dahliae infection than the wild type. Protein extraction of transgenic tobacco showed strong antifungal activity against V. dahliae, indicating that StoCYP77A2 may be involved in the synthesis of some antimicrobial compounds. These results suggest that StoCYP77A2 plays a role in plant defense to V. dahliae infection.