Isolation and characterization of novel microsatellite markers for Bletilla striata and inter-specific amplification in 2 congeneric species

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• 作者：Yusi Zhang ; Beiwei Hou ; Weiming Zhang ; Xiaoyu Ding
• 关键词：Bletilla striata ; Microsatellites ; Biotin鈥搒treptavidin capture method ; Genetic diversity ; Conservation
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：7
• 期：2
• 页码：483-485
• 全文大小：308 KB
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• 作者单位：Yusi Zhang (1)
  Beiwei Hou (2)
  Weiming Zhang (2)
  Xiaoyu Ding (1)
  
  1. College of Life Sciences, Nanjing Normal University, No. 1, Wenyuan Road, Nanjing, 210023, China
  2. Nanjing Institute for Comprehensive Utilization of Wild Plants, No. 4, Jiangwangmiao Road, Nanjing, 210042, China
  
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

Bletilla striata (Orchidaceae) is an endangered plant species with high ornamental and medicinal value. It is important to acquaint the molecular proofs to protect this endangered species. To evaluate the genetic diversity and population structure of B. striata, 10 polymorphic microsatellites were developed from microsatellite-enriched libraries. Microsatellite loci polymorphism was tested across a total of 31 individuals. The number of alleles per locus both ranged from 4 to 11, with an average of 7.5 per locus. The observed and expected heterozygosities ranged from 0.3077 to 0.6923 and from 0.4908 to 0.8153, respectively. Cross-species amplification of all loci was successful among two congeneric species. The 10 microsatellite loci identified in this study can be utilized as novel markers for further investigation of conservation genetics of B. striata and its congeneric species.