Safety assessment of lepidopteran insect-protected transgenic rice with cry2A* gene

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• 作者：Shiying Zou ; Kunlun Huang ; Wentao Xu ; Yunbo Luo ; Xiaoyun He
• 关键词：Genetically modified rice ; Cry2A* gene ; Safety assessment ; 90 ; Day feeding study
• 刊名：Transgenic Research
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：25
• 期：2
• 页码：163-172
• 全文大小：557 KB
• 参考文献：Barton KA, Whiteley HR, Yang NS (1987) Bacillus thuringiensis section sign-endotoxin expressed in transgenic Nicotiana tabacum provides resistance to lepidopteran insects. Plant Physiol 85:1103–1109PubMedCentral CrossRef PubMed
  Betz FS, Hammond BG, Fuchs RL (2000) Safety and advantages of Bacillus thuringiensis-protected plants to control insect pests. Regul Toxicol Pharmacol 32:156–173CrossRef PubMed
  Cao S, He X, Xu W, Luo Y, Yuan Y, Liu P, Cao B, Shi H, Huang K (2012) Safety assessment of transgenic Bacillus thuringiensis rice T1c-19 in Sprague–Dawley rats from metabonomics and bacterial profile perspectives. IUBMB Life 64:242–250CrossRef PubMed
  Chen H, Tang W, Xu C, Li X, Lin Y, Zhang Q (2005) Transgenic indica rice plants harboring a synthetic cry2A* gene of Bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests. Theor Appl Genet 111:1330–1337CrossRef PubMed
  Cohen M, Gould F, Bentur J (2000) Bt rice: practical steps to sustainable use. Int Rice Res Notes 25:4–10
  Estruch JJ, Carozzi NB, Desai N, Duck NB, Warren GW, Koziel MG (1997) Transgenic plants: an emerging approach to pest control. Nat Biotechnol 15:137–141CrossRef PubMed
  Karim S, Dean DH (2000) Toxicity and receptor binding properties of Bacillus thuringiensis delta-endotoxins to the midgut brush border membrane vesicles of the rice leaf folders, Cnaphalocrocis medinalis and Marasmia patnalis. Curr Microbiol 41:276–283CrossRef PubMed
  Kuiper HA, Kleter GA, Noteborn HP, Kok EJ (2001) Assessment of the food safety issues related to genetically modified foods. Plant J 27:503–528CrossRef PubMed
  Larkin P, Harrigan GG (2007) Opportunities and surprises in crops modified by transgenic technology: metabolic engineering of benzylisoquinoline alkaloid, gossypol and lysine biosynthetic pathways. Metabolomics 3:371–382CrossRef
  Manimaran P, Ramkumar G, Mohan M, Mangrauthia SK, Padmakumari AP, Muthuraman P, Bentur JS, Viraktamath BC, Balachandran SM (2011) Bt rice evaluation and deployment strategies. GM Crops 2:135–137CrossRef PubMed
  Matteson PC (2000) Insect pest management in tropical Asian irrigated rice. Annu Rev Entomol 45:549–574CrossRef PubMed
  Mendelsohn M, Kough J, Vaituzis Z, Matthews K (2003) Are Bt crops safe? Nat Biotechnol 21:1003–1009CrossRef PubMed
  OECD (1993a) Safety evaluation of foods derived by modern biotechnology. Organisation for Economic Cooperation and Development, Paris. http://​oecd.​org/​dsti/​sti/​s_​t/​biotech/​prod/​modernhtm
  OECD (1996) Food safety evaluation. Organisation for Economic Corporation and Development, Paris
  OECD (1998) Report of the OECD workshop on the toxicological and nutritional testing of novel foods, Aussois, France, 5–8 March 1997. Organisation for Economic Cooperation and Development, Paris. http://​oecd.​org/​ehs/​ehsmono/​aussoidrEN.​pdf
  Oerke E-C, Dehne H-W, Schönbeck F, Weber A (2012) Crop production and crop protection: estimated losses in major food and cash crops. Elsevier, Amsterdam
  Qi X, He X, Luo Y, Li S, Zou S, Cao S, Tang M, Delaney B, Xu W, Huang K (2012) Subchronic feeding study of stacked trait genetically-modified soybean (3O5423 x 40-3-2) in Sprague–Dawley rats. Food Chem Toxicol 50:3256–3263CrossRef PubMed
  Reeves PG, Rossow KL, Lindlauf J (1993) Development and testing of the AIN-93 purified diets for rodents: results on growth, kidney calcification and bone mineralization in rats and mice. J Nutr 123:1923–1931PubMed
  Seck PA, Diagne A, Mohanty S, Wopereis MC (2012) Crops that feed the world 7: rice. Food Secur 4:7–24CrossRef
  Tyagi AK, Mohanty A (2000) Rice transformation for crop improvement and functional genomics. Plant Sci 158:1–18CrossRef PubMed
  Wang Z, Wang Y, Cui H, Xia Y, Altosaar I, Shu Q (2002) Toxicological evaluation of transgenic rice flour with a synthetic cry1Ab gene from Bacillus thuringiensis. J Sci Food Agric 82:738–744CrossRef
  Wang EH, Yu Z, Hu J, Jia XD, Xu HB (2014) A two-generation reproduction study with transgenic Bt rice TT51 in Wistar rats. Food Chem Toxicol 65:312–320CrossRef PubMed
  Yoshikawa Y, Kishimoto Y, Tagami H, Kanahori S (2013) Assessment of the safety of hydrogenated resistant maltodextrin: reverse mutation assay, acute and 90-day subchronic repeated oral toxicity in rats, and acute no-effect level for diarrhea in humans. J Toxicol Sci 38:459–470CrossRef PubMed
  Yuan Y, Xu W, He X, Liu H, Cao S, Qi X, Huang K, Luo Y (2013) Effects of genetically modified T2A-1 rice on the GI health of rats after 90-day supplement. Sci Rep 3:1962PubMedCentral PubMed
  Zhang M, Zhuo Q, Tian Y, Piao J, Yang X (2014) Long-term toxicity study on transgenic rice with Cry1Ac and sck genes. Food Chem Toxicol 63:76–83CrossRef PubMed
  Zhu Y, He X, Luo Y, Zou S, Zhou X, Huang K, Xu W (2013) A 90-day feeding study of glyphosate-tolerant maize with the G2-aroA gene in Sprague–Dawley rats. Food Chem Toxicol 51:280–287CrossRef PubMed
  Zou S, He X, Liu Y, Chen D, Luo Y, Huang K, Zhang W, Xu W (2014) Toxicological evaluation of lactase derived from recombinant Pichia pastoris. PLoS One 9:e106470PubMedCentral CrossRef PubMed
  
• 作者单位：Shiying Zou (1) (2)
  Kunlun Huang (1) (2) (3)
  Wentao Xu (1) (2) (3)
  Yunbo Luo (1) (2)
  Xiaoyun He (1) (2) (3)
  
  1. College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing, 100083, China
  2. The Supervision, Inspection and Testing Center for Genetically Modified Organisms, Ministry of Agriculture, Beijing, 100083, China
  3. Beijing Laboratory of Food Quality and Safety, Beijing, 100083, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Molecular Medicine
  Plant Genetics and Genomics
  Animal Genetics and Genomics
  Plant Sciences
  Human Genetics
  
• 出版者：Springer Netherlands
• ISSN：1573-9368

文摘

Numerous genetically modified (GM) crops expressing proteins for insect resistance have been commercialized following extensive testing demonstrating that the foods obtained from them are as safe as that obtained from their corresponding non-GM varieties. In this paper, we report the outcome of safety studies conducted on a newly developed insect-resistant GM rice expressing the cry2A* gene by a subchronic oral toxicity study on rats. GM rice and non-GM rice were incorporated into the diet at levels of 30, 50, and 70 % (w/w), No treatment-related adverse or toxic effects were observed based on an examination of the daily clinical signs, body weight, food consumption, hematology, serum biochemistry, and organ weight or based on gross and histopathological examination. These results demonstrate that the GM rice with cry2A* gene is as safe for food as conventional non-GM rice.