australe monosomic alien chromosome addition lines (MAAL) using a combination of morphological survey, microsatellite marker-assisted selection, and molecular cytogenetic analysis. The amphidiploid (2n?=?78, AADDGG) of G. australe and G. hirsutum was consecutively backcrossed with upland cotton to develop alien addition lines of individual G. australe chromosomes in G. hirsutum. From these backcross progeny, we generated the first complete set of chromosome addition lines in cotton; 11 of 13 lines are monosomic additions, and chromosomes 7Ga and 13Ga are multiple additions. MAALs of 1Ga and 11Ga were the first to be isolated. The chromosome addition lines can be employed as bridges for the transfer of desired genes from G. australe into G. hirsutum, as well as for gene assignment, isolation of chromosome-specific probes, flow sorting and microdissection of chromosome, development of chromosome-specific ‘‘paints’-for fluorochrome-labeled DNA fragments, physical mapping, and selective isolation and mapping of cDNAs for a particular G. australe chromosome." />
Construction of a complete set of alien chromosome addition lines from Gossypium australe in Gossypium hirsutum: morphological, cytological, and genotypic characterization
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  • 作者：Yu Chen (1)
    Yingying Wang (1)
    Kai Wang (1)
    Xiefei Zhu (1)
    Wangzhen Guo (1)
    Tianzhen Zhang (1)
    Baoliang Zhou (1)
  • 刊名：Theoretical and Applied Genetics
  • 出版年：2014
  • 出版时间：May 2014
  • 年：2014
  • 卷：127
  • 期：5
  • 页码：1105-1121
  • 全文大小：
  • 参考文献：1. Ahoton L, Lacape JM, Baudoin JP, Mergeai G (2003) Introduction of Australian diploid cotton genetic variation into upland cotton. Crop Sci 43:1999-005 CrossRef
    2. Ali SNH, Ramanna MS, Jacobsen E, Visser RGF (2001) Establishment of a complete series of a monosomic tomato chromosome addition lines in the cultivated potato using RFLP and GISH analyses. Theor Appl Genet 103:687-95 CrossRef
    3. Altman DW, Stelly DM, Kohel RJ (1987) Introgression of glanded-plant and glandless-seed trait from Gossypium sturtianum Willis into cultivated upland cotton using ovule culture. Crop Sci 27(5):880-84 CrossRef
    4. Bao WK, Leng L (2005) Determination methods for photosynthetic pigment content of bryophyte with special relation of extracting solvents. Chin J Appl Environ Biol 11:235-37 (in Chinese with English abstract)
    5. Bento M, Gustafson P, Viegas W, Silva M (2010) Genome merger: from sequence rearrangements in triticale to their elimination in wheat-rye addition lines. Theor Appl Genet 121:489-97 CrossRef
    6. Bi IV, Baudoin JP, Mergeai G (1998) Cytogenetics of the ‘gland-less-seed and glanded-plant-trait from / Gossypium sturtianum Willis introgressed into upland cotton ( / Gossypium hirsutum L.). Plant Breed 117(3):235-41 CrossRef
    7. Bi IV, Baudoin JP, Mergeai G (1999a) Development of high-gossypol cotton plants with low-gossypol seeds using trispecies bridge crosses and in vitro culture of seed embryos. Euphytica 106(3):243-51 CrossRef
    8. Bi IV, Maquet A, Baudoin JP, Jardin PD, Jacquemin JM, Mergeai G (1999b) Breeding for “low-gossypol seed and high-gossypol plants-in upland cotton. Analysis of tri-species hybrids and backcross progenies using AFLPs and mapped RFLPs. Theor Appl Genet 99:1233-244 CrossRef
    9. Bottger GT, Sheehan ET, Lukefahr MJ (1964) Relation of gossypol content of cotton plants to insect resistance. J Econ Entomol 57:283-85
    10. Brubaker CL, Brown AHD (2003) The use of multiple alien chromosome addition aneuploids facilitates genetic linkage mapping of the / Gossypium G genome. Genome 46:774-91 CrossRef
    11. Budahn H, Schrader O, Peterka H (2008) Development of a complete set of disomic rape-radish chromosome-addition lines. Euphytica 162:117-28 CrossRef
    12. Chen BY, Simonsen V, Lannrr-Herrera C, Heneen WK (1992) A / Brassica campestris- / alboglabra addition line and its use for gene mapping, intergenomic gene transfer and generation of trisomics. Theor Appl Genet 84:592-99
    13. Chen CC, Chen SK, Liu MC, Kao YY (2002) Mapping of DNA markers to arms and sub-arm regions of / Nicotiana sylvestris chromosomes using aberrant alien addition lines. Theor Appl Genet 105:8-5 CrossRef
    14. Chen JF, Luo XD, Qian CT, Jahn MM, Staub JE, Zhuang FY, Lou QF, Ren G (2004) / Cucumis monosomic alien addition lines: morphological, cytological, and genotypic analyses. Theor Appl Genet 108:1343-348 CrossRef
    15. Cifuentes M, Benavente E (2009) Wheat-alien metaphase I pairing of individual wheat genomes and D genome chromosomes in interspecific hybrids between / Triticum aestivum L. and / Aegilops geniculata Roth. Theor Appl Genet 119:805-13 CrossRef
    16. Dilday RH (1986) Development of a cotton plant with glandless seeds and glanded foliage and fruiting forms. Crop Sci 26:639-41 CrossRef
    17. Endrizzi JE, Turcotte EL, Kohel RJ (1985) Genetics, cytology, and evolution of / Gossypium. Adv Genet 23:271-75 CrossRef
    18. Fang XH, Gu SH, Xu ZY, Chen F, Guo DD, Zhang HB, Wu NH (2004) Construction of a binary BAC library for an apomictic monosomic addition line of / Beta corolliflora in sugar beet and identification of the clones derived from the alien chromosome. Theor Appl Genet 108:1420-425 CrossRef
    19. Friebe B, Qi LL, Nasuda S, Zhang P, Tuleen NA, Gill BS (2000) Development of a complete set of / Triticum aestivum- / Aegilops speltoides chromosome addition lines. Theor Appl Genet 101:51-8 CrossRef
    20. Fryxell PA (1992) A revised taxonomic interpretation of / Gossypium L. (Malvaceae). Rheedea 2:108-65
    21. Fu S, Lv Z, Qi B, Guo X, Jun Li, Liu B, Han F (2012) Molecular Cytogenetic characterization of wheat- / Thinopyrum elongatum addition, substitution and translocation lines with a novel source of resistance to wheat / Fusarium head blight. J Genet Genomics 39:103-10 CrossRef
    22. Gao D, Guo D, Jung C (2001) Monosomic addition lines of / Beta corolliflora Zoss in sugar beet: cytological and molecular-marker analysis. Theor Appl Genet 103:240-47 CrossRef
    23. Geleta M, Heneen WK, Stoute AI, Muttucumaru N, Scott RJ, King GJ, Kurup S, Bryngelsson T (2012) Assigning / Brassica microsatellite markers to the nine C-genome chromosomes using / Brassica rapa var. / trilocularis-em class="a-plus-plus">B. oleracea var. / alboglabra monosomic alien addition lines. Theor Appl Genet 125:455-66 CrossRef
    24. Gerstel DU, Sarvella PA (1956) Additional observations on chromosomal translocations in cotton hybrids. Evolution 10:408-14 CrossRef
    25. Guan B, Wang K, Zhou BL, Guo WZ, Zhang TZ (2008) Establishment of a multi-color genomic in situ hybridization technique to simutaneously discriminated the three interspecific hybrid genomes in / Gossypium. J Integr Plant Biol 50:345-51 CrossRef
    26. Guo WZ, Wang W, Zhou BL, Zhang TZ (2006) Cross-species transferability of / G. arboreum-derived EST-SSRs in the diploid species of / Gossypium. Theor Appl Genet 112:1573-581 CrossRef
    27. Guo WZ, Cai CP, Wang CB, Han ZG, Song XL, Wang K, Niu XW, Wang C, Lu KY, Shi B, Zhang TZ (2007) A microsatellite-based, gene-rich linkage map reveals genome structure, function and evolution in / Gossypium. Genetics 176:527-41 CrossRef
    28. Harlan JR, de Wart JMJ (1971) Toward rational classification of cultivated plants. Taxon 20:509-17 CrossRef
    29. Hau B (1981) Lignées d’addition sur l’espèce / Gossypium hirsutum L. I. Utilisation de l’hybridation interspécifique et de la method des lignées d’addition pour l’amélioration du cotonnier. Cot Fib Trop 36:247-58
    30. He JX, Sun CW (1994) A scheme for introgression of delayed gland morphogenesis gene from wild / Gossypium bickii into cultivated upland cotton ( / G. hirsutum). Acta Genetica Sinica 21(1):52-8
    31. He JX, Jiang RQ, Zhang XX, Li ZS, Zhao GZ (2000) Genetic analysis for introgressive genes of petal color from / Gossypium bickii to / G. hirsutum. Acta Genetica Sinica 27(4):344-50
    32. Ji Y, Chetelat RT (2003) Homoeologous pairing and recombination in / Solanum lycopersicoides monosomic addition and substitution lines of tomato. Theor Appl Genet 106:979-89
    33. Jiang CX, Chee PW, Draye X, Morrell PL, Smith CW, Paterson AH (2000) Multilocus interactions restrict gene introgression in interspecific populations of polyploid / Gossypium (cotton). Evolution 54:798-14 CrossRef
    34. Jiang SM, Hu J, Yin WB, Chen YH, Wang RRC, Hu ZM (2005) Cloning of resistance gene analogs located on the alien chromosome in an addition line of wheat- / Thinopyrum intermedium. Theor Appl Genet 111:923-31 CrossRef
    35. Kishii M, Yamada T, Sasakuma T, Tsujimoto H (2004) Production of wheat-em class="a-plus-plus">Leymus racemosus chromosome addition lines. Theor Appl Genet 109:255-60 CrossRef
    36. Kong F, Wang H, Cao A, Qin B, Ji J, Wang S, Wang X (2008) Characterization of / T. aestivum- / H. californicum chromosome addition lines DA2H and MA5H. J Genet Genomics 35:673-78 CrossRef
    37. Kulkarni VN, Khadi BM, Sangam VS (2002) Pre-breeding efforts for low gossypol seed and high gossypol plant in / G. herbaceum L. cotton utilizing / G. australe Mueller. Curr Sci 82(4):434-39
    38. Kynast RG, Riera-Lizarazu O, Vales MI, Okagaki RJ, Maquieira S, Chen G, Ananiev EV, Odland WE, Russell CD, Stec AO, Livingston SM, Zaia HA, Rines HW, Phillips RL (2001) A complete set of maize individual chromosome additions to the oat genome. Plant Physiol 125:1216-227 CrossRef
    39. Kynast RG, Okagaki RJ, Galatowitsch MW, Granath SR, Jacobs MS, Stec AO, Rines HW, Phillips RL (2004) Dissecting the maize genome by using chromosome addition and radiation hybrid lines. Proc Natl Acad Sci USA 101:9921-926 CrossRef
    40. Liang ZL, Jiang RQ, Zhong WN (1997) New red flower germplasm lines of cotton selected from hybrid of / Gossypium hirsutum?×? / G. bickii. Sci China (series C) 40:284-92 CrossRef
    41. Lopez-Lavalle LAB, Brubaker CL (2007) Frequency and fidelity of alien chromosome transmission in / Gossypium hexaploid bridging population. Genome 50:479-91 CrossRef
    42. McMichael SC (1960) Combined effects of glandless genes / gl / 2 and / gl / 3 on pigment glands in the cotton plant. Agron J 52:385-86 CrossRef
    43. Mergeai G (1992) New perspectives concerning the methodology to be used for introgression of the glanded plant and glandless seed character in cultivated cotton ( / Gossypium hirsutum L.). Cot Fib Trop 47:113-18
    44. Molnár I, Molnár -Láng M (2010) GISH reveals different levels of meiotic pairing with wheat for individual / Aegilops biuncialis chromosomes. Bilo Plant 54:259-64 CrossRef
    45. Multani DS, Khush GS, delos Reyes BG, Brar DS (2003) Alien genes introgression and development of monosomic alien addition lines from / Oryza latifolia Desv. to rice Oryza sativa L. Theor Appl Genet 107:395-05 CrossRef
    46. Muramoto H (1969) Hexaploid cotton: some plant and fiber properties. Crop Sci 9:27-9 CrossRef
    47. Ozkan H, Levy AA, Feldman M (2001) Allopolyploidy induced rapid genome evolution in the wheat ( / Aegilops-em class="a-plus-plus">Triticum) group. Plant Cell 13:1735-747 CrossRef
    48. Paterson AH, Brubaker KL, Wendel JF (1993) A rapid method for extraction of cotton ( / Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Rep 11:122-27 CrossRef
    49. Pertuzé RA, Ji Y, Chetelat RT (2003) Transmission and recombination of homeologous / Solanum sitiens chromosomes in tomato. Theor Appl Genet 107:1391-401 CrossRef
    50. Peterka H, Budahn H, Schrader O, Ahne R, Schutze W (2004) Transfer of resistance against the beet cyst nematode from radish ( / Raphanus sativus) to rape ( / Brassica napus) by monosomic chromosome addition. Theor Appl Genet 109:30-1 CrossRef
    51. Reamon-Ramos SM, Wricke G (1992) A full set of monosomie addition lines in / Beta vulgaris from / Beta webbiana: morphology and isozyme markers. Theor Appl Genet 84:411-18
    52. Rooney WL, Stelly DM (1991) Identification of four / Gossypium monosomic alien addition derivatives from a backcrossing program with / G. hirsutum. Crop Sci 31:337-41 CrossRef
    53. Sarr D, Lacape JM, Rodier-Goud M, Jacquemin JM, Benbouza H, Toussaint A, Palm R, Ahoton L, Baudoin JP, Mergeai G (2011) Isolation of five new monosomic alien addition lines of / Gossypium australe F. Muell in / G. hirsutum L. by SSR and GISH analyses. Plant Breed 130:60-6 CrossRef
    54. Sears ER (1954) The aneuploids of common wheat. Univ Mo Res Bull 572:1-8
    55. Shaked H, Kashkush K, Ozkan H, Feldman M, Levy AA (2001) Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. Plant Cell 13:1749-759 CrossRef
    56. Shim J, Panaud O, Vitte C, Mendioro MS, Brar DS (2010) RDA derived / Oryza minuta-specific clones to probe genomic conservation across / Oryza and introgression into rice ( / O. sativa L.). Euphytica 176:269-79 CrossRef
    57. Skalicka K, Lim KY, Matyasek R, Matzke M, Leitch AR, Kovarik A (2005) Preferential elimination of repeated DNA sequences from the paternal, / Nicotiana tomentosiformis genome donor of a synthetic, allotetraploid tobacco. New Phytol 166:291-03 CrossRef
    58. Srinivasan K, Malathi VG, Kirti PB, Prakash S, Chopra VL (1998) Generation and characterisation of monosomic chromosome addition lines of / Brassica campestris- / B. oxyrrhina. Theor Appl Genet 97:976-81 CrossRef
    59. Stewart, JM (1995) Potential for crop improvement with exotic germplasm and genetic engineering. In: Constable GA, Forrester NW (eds) Challenging the future: Proceedings of the World Cotton Research Conference-1, Brisbane Australia, February 14-7, pp 313-27, Melbourne, Australia
    60. Wang LM, Wang JB (2008) The utilization of HB-red flower in hybrid cotton breeding. Cotton Sci 20:86
    61. Wang P, Zhang TZ (2012) Genetic dissection of photosynthetic pigment content in cotton interspecific chromosome segment introgression lines. Acta Agronomica Sinica 38:947-53 CrossRef
    62. Wang XE, Chen PD, Liu DJ, Zhang P, Zhou B, Friebe B, Gill BS (2001) Molecular cytogenetic characterization of / Roegneria ciliaris chromosome additions in common wheat. Theor Appl Genet 102:651-57 CrossRef
    63. Wang K, Song XL, Han ZG, Guo WZ, Yu JZ, Sun J, Pan JJ, Kohel RJ, Zhang TZ (2006) Complete assignment of the chromosomes of / Gossypium hirsutum L. by translocation and fluorescence in situ hybridization mapping. Theor Appl Genet 113:73-0 CrossRef
    64. Wendel JF, Cronn RC (2003) Polyploidy and the evolutionary history of cotton. Adv Agron 78:139-86 CrossRef
    65. Zhang BJ, Li BL, Wan LM, Han YH, Wang RH, Zhu SJ, Wang HM (1994) Synthesis of allotetraploid cotton with [AG] complex chromosome set. Chin Sci Bull 39(7):644-48
    66. Zhang J, Wu YT, Guo WZ, Zhang TZ (2000) Fast screening of microsatellite markers in cotton with PAGE/silver staining. Cotton Sci Sin 12:267-69
    67. Zhang J, Guo WZ, Zhang TZ (2002) Molecular linkage map of allotetraploid cotton ( / Gossypium hirsutum L.?×? / Gossypium barbadense L.) with a haploid population. Theor Appl Genet 105:1166-174 CrossRef
    68. Zhou ZH, Yu P, Liu GH, He JX, Chen JX, Zhang XX (2004) Morphological and molecular characterization of two / G. somalense monosomic alien addition lines (MAALs). Chin Sci Bull 49:558-62
    69. Zhu SJ, Jiang YR, Reddy N, Ji DF (2004) Breeding, introgression and inheritance of delayed gland morphogenesis trait from / Gosspium bickii into upland cotton germplasm. Chin Sci Bull 49:2470-476
  • 作者单位：Yu Chen (1)
    Yingying Wang (1)
    Kai Wang (1)
    Xiefei Zhu (1)
    Wangzhen Guo (1)
    Tianzhen Zhang (1)
    Baoliang Zhou (1)

    1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China
  • ISSN：1432-2242
文摘
Key message We report the first complete set of alien addition lines of G. hirsutum . The characterized lines can be used to introduce valuable traits from G. australe into cultivated cotton. Abstract Gossypium australe is a diploid wild cotton species (2n?=?26, GG) native to Australia that possesses valuable characteristics unavailable in the cultivated cotton gene pool, such as delayed pigment gland morphogenesis in the seed and resistances to pests and diseases. However, it is very difficult to directly transfer favorable traits into cultivated cotton through conventional gene recombination due to the absence of pairing and crossover between chromosomes of G. australe and Gossypium hirsutum (2n?=?52, AADD). To enhance the transfer of favorable genes from wild species into cultivated cotton, we developed a set of hirsutum-em class="a-plus-plus">australe monosomic alien chromosome addition lines (MAAL) using a combination of morphological survey, microsatellite marker-assisted selection, and molecular cytogenetic analysis. The amphidiploid (2n?=?78, AADDGG) of G. australe and G. hirsutum was consecutively backcrossed with upland cotton to develop alien addition lines of individual G. australe chromosomes in G. hirsutum. From these backcross progeny, we generated the first complete set of chromosome addition lines in cotton; 11 of 13 lines are monosomic additions, and chromosomes 7Ga and 13Ga are multiple additions. MAALs of 1Ga and 11Ga were the first to be isolated. The chromosome addition lines can be employed as bridges for the transfer of desired genes from G. australe into G. hirsutum, as well as for gene assignment, isolation of chromosome-specific probes, flow sorting and microdissection of chromosome, development of chromosome-specific ‘‘paints’-for fluorochrome-labeled DNA fragments, physical mapping, and selective isolation and mapping of cDNAs for a particular G. australe chromosome.

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