Overexpression of a PLD¦Á1 gene from Setaria italica enhances the sensitivity of Arabidopsis to abscisic acid and improves its drought tolerance

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• 作者：Yunling Peng ; Jinpeng Zhang ; Gaoyi Cao ; Yuanhong Xie ; Xihui Liu ; Minhui Lu and Guoying Wang
• 关键词：Setaria italica ; PLDα ; 1 ; ABA sensitivity ; Drought tolerance
• 刊名：Plant Cell Reports
• 出版年：2010
• 出版时间：July, 2010
• 年：2010
• 卷：29
• 期：7
• 页码：793-802
• 全文大小：839.8 KB

文摘

Phospholipase D (PLD) plays an important role in various physiological processes in plants, including drought tolerance. Here, we report the cloning and characterization of the full-length cDNA of PLDα1 from foxtail millet, which is a cereal crop with high water use efficiency. The expression pattern of the SiPLDα1 gene in foxtail millet revealed that it is up-regulated under dehydration, ABA and NaCl treatments. Heterologous overexpression of SiPLDα1 in Arabidopsis can significantly enhance their sensitivity to ABA, NaCl and mannitol during post-germination growth. Under water deprivation, overexpression of SiPLDα1 in Arabidopsis resulted in significantly enhanced tolerance to drought stress, displaying higher biomass and RWC, lower ion leakage and higher survival percentages than the wild type. Further analysis indicated that transgenic plants showed increased transcription of the stress-related genes, RD29A, RD29B, RAB18 and RD22, and the ABA-related genes, ABI1 and NCED3 under dehydration conditions. These results demonstrate that SiPLDα1 is involved in plant stress signal transduction, especially in the ABA signaling pathway. Moreover, no obvious adverse effects on growth and development in the 35S::SiPLDα1 transgenic plants implied that SiPLDα1 is a good candidate gene for improving crop drought tolerance.