Downregulation of galectin-3 by EGF mediates the apoptosis of HepG2 cells

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• 作者：Zhenzhen Hu (1) ansel.zzhu@gmail.com
  Xiuqin Jiang (1) jiangxiuq@gmail.com
  Ying Xu (1) xuying4977@gmail.com
  Nan Lu (2) lexie0423@gmail.com
  Weizhi Wang (3) wwz1987house@gmail.com
  Jie Luo (1) luojie2101@gmail.com
  Han Zou (3) 493040059@qq.com
  Datong Zheng (14) dtzheng@njmu.edu.cn
  Xing Feng (5) xing_feng66@hotmail.com
• 关键词：Epidermal growth factor receptor – ; Protein kinase B – ; Extracellular regulated protein kinases – ; Galectin ; 3 – ; Apoptosis
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：369
• 期：1-2
• 页码：157-165
• 全文大小：950.7 KB
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• 作者单位：1. The Research Core, Second Hospital, The Second Clinical School, Nanjing Medical University, 121 Jiangjiayuan Street, Nanjing, 210011 Jiangsu, China2. Fudan University, 220 Handan Street, Shanghai, 200433 China3. The First Clinical School, Nanjing Medical University, 300 Guangzhou Street, Nanjing, 210029 Jiangsu, China4. The Research Core and Department of Pediatrics, Second Hospital, The Second Clinical School, Nanjing Medical University, Room 601, Building 1, 121 Jiangjiayuan Street, Nanjing, 210011 Jiangsu, China5. Department of Neonatology, Children鈥檚 Hospital of Suzhou University, Suzhou, 215003 Jiangsu, China
• ISSN：1573-4919

文摘

Epidermal growth factor (EGF) in high concentrations induces apoptosis of the tumor cells which express high levels of epidermal growth factor receptor. However, the precise mechanism for this induction is not clear. Galectin-3 is the most probable candidate for mediating this effect, as it is known to induce anti-apoptotic activity in a variety of tumor cells exposed to diverse apoptotic stimuli. In this study, we determined whether galectin-3 plays a role in high concentrations of EGF-induced apoptosis of HepG2 cells. We found that EGF in high concentrations led to the growth inhibition of HepG2 cells, which were associated with promotion of cell death. High concentrations of EGF suppressed cytoplasmic expression of galectin-3. Moreover, we demonstrated overexpression of galectin-3 could reduce EGF-induced apoptosis in HepG2 cells. Our study demonstrated for the first time that downregulation of cytoplasmic galectin-3 was essential for high concentrations of EGF-induced apoptosis in HepG2 cells.