Polymorphism of human cytomegalovirus (HCMV) UL144 gene in low passage clinical isolates

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• 作者：Xingxing Fu (1)
  Liping Li (2)
  Chaoyang Huang (3)
  Xiaoman Li (3)
  Huadong Wang (4)
  Zhenyuan Xie (5)
  Ze Chen (1) (3) (4)
  
• 关键词：Human cytomegalovirus ; Gene polymorphism ; UL144 gene ; Glycoprotein B (UL55) gene ; Virus virulence
• 刊名：Chinese Science Bulletin
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：59
• 期：25
• 页码：3120-3126
• 全文大小：519 KB
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• 作者单位：Xingxing Fu (1)
  Liping Li (2)
  Chaoyang Huang (3)
  Xiaoman Li (3)
  Huadong Wang (4)
  Zhenyuan Xie (5)
  Ze Chen (1) (3) (4)
  
  1. Shanghai Institute of Biological Products, Shanghai, 200052, China
  2. Institute of Pediatric Research, Hunan Children鈥檚 Hospital, Changsha, 410007, China
  3. Laboratory of Molecular Virology, School of Life Science, Hunan Normal University, Changsha, 410084, China
  4. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China
  5. Xie Tu Community Medical Service Center, Shanghai, 200032, China
  
• ISSN：1861-9541

文摘

To explore the impact of gene polymorphism of human cytomegalovirus (HCMV) on virus virulence, the full-length UL144 gene and partial sequence of glycoprotein B (UL55) gene were sequenced and analyzed for 23 clinical strains of HCMV isolated from urine samples of pediatric patients with congenital or postnatal HCMV infection. Among the 23 isolates, 13 (57 %) were UL144 genotype 1A, 3(13 %) were UL144 genotypes 2 and 7(30 %) were UL144 genotype 3; geographic differences in genotype distribution were found for both UL144 and gB gene. No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US. Our results also demonstrated that for all clinical strains of gB genotypes I, III and UL144 genotypes 1A, 2, and 3 found in this study, mother-to-fetus vertical transmission was possible.