Antibacterial effect of different extracts from Wikstroemia indica on Escherichia coli based on microcalorimetry coupled with agar dilution method

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• 作者：Chang Chen ; Fen Qu ; Jian Wang ; Xinhua Xia…
• 关键词：Wikstroemia indica ; Anti ; E. coli ; Bioactive fraction ; Microcalorimetry ; Agar dilution method
• 刊名：Journal of Thermal Analysis and Calorimetry
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：123
• 期：2
• 页码：1583-1590
• 全文大小：1,220 KB
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• 作者单位：Chang Chen (1) (2)
  Fen Qu (3)
  Jian Wang (1)
  Xinhua Xia (4)
  Jiabo Wang (5)
  Zhe Chen (1) (2)
  Xiao Ma (1) (2)
  Shizhang Wei (1) (2)
  Yaming Zhang (5)
  Jianyu Li (6)
  Man Gong (6)
  Ruilin Wang (6)
  Honghong Liu (6)
  Zhirui Yang (1) (2)
  Yonggang Li (6)
  Yanling Zhao (2)
  Xiaohe Xiao (5)
  
  1. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China
  2. Department of Pharmacy, 302 Hospital of People’s Liberation Army, Beijing, 100039, China
  3. Clinical Examination Center, 302 Hospital of People’s Liberation Army, Beijing, 100039, China
  4. College of Pharmacy, Hunan University of Traditional Chinese Medicine, Hunan, 410007, China
  5. China Military Institute of Chinese Medicine, 302 Hospital of People’s Liberation Army, Beijing, 100039, China
  6. Department of Integrative Medical Center, 302 Hospital of People’s Liberation Army, Beijing, 100039, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Sciences
  Polymer Sciences
  Physical Chemistry
  Inorganic Chemistry
  Measurement Science and Instrumentation
  
• 出版者：Akad茅miai Kiad贸, co-published with Springer Science+Business Media B.V., Formerly Kluwer Academic
• ISSN：1572-8943

文摘

The root of Wikstroemia indica has been widely used in China as folk medicine for the treatment for arthritis, whooping cough, cancer, and bacillosis. However, the constituents which have antibacterial activity were not clarified yet. In this study, the antibacterial effect of five extracts from W. indica on Escherichia coli was evaluated by microcalorimetry coupled with agar dilution method. The ethanol extract of W. indica was isolated with organic solvents of different polarities including petroleum (P.E.) extract, chloroform (CHCl₃) extract, ethyl acetate (EtOAc) extract, n-butylalcohol (nBuOH) extract, and residue extract. The metabolic profiles of E. coli growth at 37 °C were measured by microcalorimetry. According to the principal component analysis, k ₁, k ₂, and P ₁ were obtained from heat flow power–time (HFP–time) curve. The agar dilution method was performed to verify the results of thermodynamics. The results of microcalorimetric experiment indicated that EtOAc fraction demonstrated the strongest antibacterial activity with half-inhibitory concentration of 92.4 μg mL−1. Meanwhile, similar results were gained from the common method of agar diffusion, which suggested that EtOAc extract could be further developed as antibacterial bioactive fraction of W. indica. Altogether, microcalorimetry is a useful technique to provide sufficient quantitative information and evaluate the antimicrobial effect with its sensitive. Keywords Wikstroemia indica Anti-E. coli Bioactive fraction Microcalorimetry Agar dilution method