Ceftiofur Regulates LPS-Induced Production of Cytokines and Improves LPS-Induced Survival Rate in Mice

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• 作者：Xinxin Ci ; Hongyu Li ; Yu Song ; Na An ; Qinlei Yu ; Fanqin Zeng and Xuming Deng
• 关键词：KEY WORDS ceftiofur ; LPS ; cytokines ; survival rate ; mice
• 刊名：Inflammation
• 出版年：2008
• 出版时间：December, 2008
• 年：2008
• 卷：31
• 期：6
• 页码：422-427
• 全文大小：117.0 KB

文摘

The influence of ceftiofur on immune responses has been suggested by results of in vitro studies. This effect was studied using a murine model that measured mortality and early cytokine responses after challenge with endotoxin. To investigate the treatment of endotoxic mice with ceftiofur, mice were pretreated with ceftiofur at different times before and after challenge with a lethal dose of 30 mg/kg lipopolysaccharide (LPS). We found that 20 mg/kg ceftiofur had a significant protective effect and reduced the mortality of mice at early stages. To further understand the mechanism of action of ceftiofur, we examined plasma cytokine levels. Mice treated with LPS alone showed markedly increased plasma levels of TNF-α, IL-1β, IL-6 and IL-10, whereas mice pretreated with 20 mg/kg ceftiofur showed significantly decreased plasma levels of TNF-α, IL-1β and IL-6, but increased plasma levels of IL-10. These results support the idea that ceftiofur has a beneficial effect on LPS-induced endotoxemia caused by LPS through its modulation of cytokine levels. This confirms the effect of ceftiofur for the treatment of endotoxemia, which is caused by a Gram-negative bacterial infection.